首页> 外国专利> Method for sequencing RNA by in-source decay using matrix assisted laser desorption ionization time of flight mass spectrometer

Method for sequencing RNA by in-source decay using matrix assisted laser desorption ionization time of flight mass spectrometer

机译:利用基质辅助激光解吸电离飞行时间质谱仪通过源内衰减对RNA进行测序的方法

摘要

An analytic method is provided for obtaining much sequence information by causing in-source decay of modified RNA and non-modified RNA and generating many fragment ions. Particularly, a method for analysis wherein a matrix that efficiently causes decomposition by in-source decay of RNA of 20 bases or longer is used in an apparatus that has a laser of a wavelength commonly used in MALDI-TOF MS. A specimen containing RNA is subjected to matrix assisted laser desorption ionization time of flight mass spectrometry that uses 2,4-dihydroxyacetophenone as a matrix to obtain fragment ions derived from the RNA. The difference in mass between the peaks of ions in the fragment ions is used to analyze the sequence of the RNA.
机译:提供了一种通过引起修饰的RNA和未修饰的RNA的源内衰变并产生许多片段离子来获得大量序列信息的分析方法。特别地,在具有在MALDI-TOF MS中通常使用的波长的激光的装置中,使用了通过在源内衰减20个碱基以上的RNA而有效地引起分解的基质的分析方法。对包含RNA的样品进行基质辅助激光解吸电离飞行时间质谱分析,该质谱法使用2,4-二羟基苯乙酮作为基质,以获得源自RNA的碎片离子。碎片离子中离子峰之间的质量差异用于分析RNA序列。

著录项

  • 公开/公告号US9040235B2

    专利类型

  • 公开/公告日2015-05-26

    原文格式PDF

  • 申请/专利权人 MASAKI YAMADA;

    申请/专利号US201113215029

  • 发明设计人 MASAKI YAMADA;

    申请日2011-08-22

  • 分类号C12Q1/68;

  • 国家 US

  • 入库时间 2022-08-21 15:19:43

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