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GENETICALLY MODIFIED CLOSTRIDIUM THERMOCELLUM ENGINEERED TO FERMENT XYLOSE

机译：经基因修饰的发酵木糖酵母

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摘要

One aspect of the invention relates to industrial bioconversion of the xylose portion of biomass materials into fuels and chemicals. Another aspect of the invention relates to industrial bioconversion of the xylan portion of biomass materials into fuels and chemicals. In one embodiment, the invention is directed to the bacterium Clostridium thermocellum, a highly cellulolytic organism that has much potential as a biocatalyst in a consolidated bioprocess configuration. In some embodiments, the invention is a genetic modification that confers the ability to ferment xylose to C. thermocellum and the strains created with this modification. In some embodiments, the genetic modification is composed of two genes contained in an operon from T. saccharolyticum. The genes express proteins with xylose isomerase (XI) and xylulokinase activites (XK). In other embodiments, the invention relates to a recombinant Clostridium thermocellum host cell capable of fermenting xylan.

机译：本发明的一个方面涉及生物质材料的木糖部分的工业生物转化为燃料和化学品。本发明的另一方面涉及生物质材料的木聚糖部分的工业生物转化为燃料和化学品。在一个实施方案中，本发明涉及一种细菌热纤梭菌（Clostridium thermocellum），该纤维素是高度纤维素分解的生物，在整合的生物过程构型中作为生物催化剂具有很大的潜力。在一些实施方案中，本发明是赋予将木糖发酵成I C的能力的遗传修饰。纤维素酶以及通过此修改创建的菌株。在一些实施方案中，遗传修饰由来自的操纵子中包含的两个基因组成。糖精。这些基因表达具有木糖异构酶（XI）和木酮糖激酶活性物（XK）的蛋白质。在其他实施方案中，本发明涉及一种能够发酵木聚糖的重组热纤梭菌宿主细胞。 展开▼

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公开/公告号US2014370561A1

专利类型

公开/公告日2014-12-18

原文格式PDF

申请/专利权人 AARON ARGYROS;TRISHA BARRETT;NICKY CAIAZZA;DAVE HOGSETT;
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申请/专利号US201113995402

发明设计人 AARON ARGYROS;TRISHA BARRETT;NICKY CAIAZZA;DAVE HOGSETT;
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申请日2011-12-22

分类号C12P7/06;C12N9/12;C12N15/74;

国家 US

入库时间 2022-08-21 15:23:40

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4. GENETICALLY MODIFIED CLOSTRIDIUM THERMOCELLUM ENGINEERED TO FERMENT XYLOSE [P] . CA2822654A1 . 2012-06-28

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5. GENETICALLY MODIFIED CLOSTRIDIUM THERMOCELLUM ENGINEERED TO FERMENT XYLOSE [P] . 世界知识产权组织专利： WO2012088467A3 . 2012-10-26

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1. Peasant Household, Government and Genetically Modified Crops --Statistic Analyses on Peasant＇ s Intentions of Planting Genetically Modified Crops in China [J] . Shuzhong, Ma 中国经济评论：英文版 . 2004,第002期

2. Application Effects of Amino Acid Powder, Fermented Rapeseed Meal and Fermented Cottonseed Meal in Feeds for AIIogynogenetic Crucian Carp [J] . KONG Li, ZHANG Wei, YE Yuan-tu, 动物与饲料科学：英文版 . 2010,第006期

3. Genetic variability for yield and fiber related traits in genetically modified cotton [J] . SAHAR Adeela, REN Maozhi, SHAKEEL Amir, 棉花研究（英文版） . 2021,第003期

4. Transcriptomic analysis of a Clostridium thermocellum strain engineered to utilize xylose: responses to xylose versus cellobiose feeding [J] . Albert E. Tafur Rangel, Trevor Croft, Andrés Fernando González Barrios, Scientific reports. . 2020,第1期

5. XYLANASE ENCODED BY GENETICALLY ENGINEERED CLOSTRIDIUM THERMOCELLUM GENE IN BACILLUS SUBTILIS [J] . Cho KH., Pack MY., Jung KH. Biotechnology Letters . 1995,第2期

6. Expression of Clostridium thermocellum endoglucanase gene in Lactobacillus gasseri and Lactobacillus johnsonii and characterization of the genetically modified probiotic Lactobacilli. [J] . Cho JaieSoon, Choi YunJaie, Chung DaeKyun, Current Microbiology: An International Journal . 2000,第4期

7. Genetically Engineered Saccharomyces cerevisiae Strain That Can Ultilize Both Xylose and Glucose for Fermentation [C] . Jingping Ge, Luyan Zhang, Wenxiang Ping, REET 2013 . 2014

8. Effect of substrate loading on metabolic flux of Clostridium thermocellum during fermentative hydrogen production using cellulosic substrates. [D] . Islam, Rumana. 2005

9. Expressing the Thermoanaerobacterium saccharolyticum pforA in engineered Clostridium thermocellum improves ethanol production [O] . Shuen Hon, Evert K. Holwerda, Robert S. Worthen, 2018

10. Acetic acid fermentability with Clostridium thermoaceticum and Clostridium thermocellum of standard compounds found in beech wood as produced in hot-compressed water [O] . Nakamura Yosuke, Miyafuji Hisashi, Kawamoto Haruo, 2011

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3. PVA浓度对固定化酵母颗粒形貌和木糖醇发酵的影响 [C] . 邓立红 ,蒋建新 ,姚思宇 . 第四届全国生物质材料科学与技术学术研讨会 . 2011

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