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SOLUBLE TREPONEMA PALLIDUM PROTEIN TP0453, TP0453-TP0326 FUSION PROTEIN, AND USE IN SYPHILIS DIAGNOSIS
SOLUBLE TREPONEMA PALLIDUM PROTEIN TP0453, TP0453-TP0326 FUSION PROTEIN, AND USE IN SYPHILIS DIAGNOSIS
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机译：可溶性白蚁蛋白TP0453，TP0453-TP0326融合蛋白及其在梅毒诊断中的应用
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摘要
The present application provides methods of producing soluble Treponema pallidum protein Tp0453 (such as the fragment shown in SEQ ID NO: 3). For example, the protein can be expressed from the pET28a vector in a cell and the resulting soluble Tp0453 protein isolated from the cell. Also provided are the isolated soluble Tp0453 protein, as well as Tp0453-Tp0326 chimeric proteins (e.g., SEQ ID NO: 11), and methods of using the proteins to detect antibodies specific for T. pallidum subsp. pallidum, for example to diagnose syphilis. Devices and kits that incorporate Tp0453 protein and Tp0453-Tp0326 chimeric proteins, such as lateral flow devices, are also disclosed. Kits that include the soluble Tp0453 protein and kits that include solid substrates containing the soluble Tp0453 protein are also provided.
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机译：本申请提供了产生可溶性梅毒螺旋体蛋白Tp0453（例如SEQ ID NO：3所示的片段）的方法。例如，可以从细胞中的pET28a载体表达该蛋白质，并从细胞中分离出所得的可溶性Tp0453蛋白质。还提供了分离的可溶性Tp0453蛋白以及Tp0453-Tp0326嵌合蛋白（例如，SEQ ID NO：11），以及使用该蛋白检测对I T特异性的抗体的方法。苍白子 subsp。例如， pallidum 用于诊断梅毒。还公开了掺入Tp0453蛋白和Tp0453-Tp0326嵌合蛋白的装置和试剂盒，例如侧向流动装置。还提供了包括可溶性Tp0453蛋白的试剂盒和包括含有可溶性Tp0453蛋白的固体底物的试剂盒。
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