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Detection of the target nucleic acid sequence using the PTO cut and extension-dependent signaling oligonucleotide cut {DetectionofTargetNucleicAcidSequencebyPTOCleavageandExtension-DependentSignalingOligonucleotideCleavage}
Detection of the target nucleic acid sequence using the PTO cut and extension-dependent signaling oligonucleotide cut {DetectionofTargetNucleicAcidSequencebyPTOCleavageandExtension-DependentSignalingOligonucleotideCleavage}
The present invention, PTO cut and extension-dependent signaling oligonucleotide cut: is related to the detection of the target nucleic acid sequence by (PTO Cleavage and Extension-Dependent Signaling Oligonucleotide Cleavage PCE-SC) assay. The present invention is done in a manner that the extended strand is produced on the CTO with any sequence as dependent templates to the presence of the target nucleic acid sequence, after all, the SO is the extended strand hybridized as a probe It will provide a signal. The present invention, PTO hybridization and cleavage, CTO hybridization and extension, and using a series of reactions containing SO hybridization and cleavage, whereby the specificity of the present invention can be improved higher. .BACKGROUND 2
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