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Selecting keratinocytes for producing three-dimensional epidermis model, comprises e.g. labeling three different biomolecules of cell homogenate of epidermis sample keratinocytes and identical biomolecules of homogenate of reference sample
Selecting keratinocytes for producing three-dimensional epidermis model, comprises e.g. labeling three different biomolecules of cell homogenate of epidermis sample keratinocytes and identical biomolecules of homogenate of reference sample
Selecting keratinocytes for producing three-dimensional epidermis model for determining skin irritation, comprises (a) providing a cell homogenate of keratinocytes of an epidermis sample, (b) labeling at least three different biomolecules, (c) labeling identical biomolecules in the cell homogenate of a reference sample, (d) determining the signal intensity of the biomolecules labeled in the steps (b) and (c), and (e) selecting the keratinocytes in which the signal intensity of at least three biomolecules labeled in the step (b) is larger than that of the biomolecules labeled in the step (c). Selecting keratinocytes for producing three-dimensional epidermis model for determining skin irritation, comprises (a) providing a cell homogenate of keratinocytes of an epidermis sample, (b) labeling at least three different biomolecules from the cell homogenate provided in the step (a), comprising (i) proteins which are involved in terminal differentiation of the keratinocytes, (ii) proteins and/or enzymes of the lipid metabolism of the keratinocytes, (iii) proteins for construction of skin barrier, (iv) proteins of cell-cell adhesion, (v) proteins which occur in inflammatory processes of the skin, or (vi) regulatory proteins which influence the metabolism of the skin, (c) labeling identical biomolecules in the cell homogenate of a reference sample, in which the keratinocytes used for preparing the cell homogenate, are selected such that they are suitable for producing three-dimensional epidermal models for toxicological in vitro tests for determining the skin irritation, (d) determining the signal intensity of the biomolecules labeled in the step (b) and the signal intensity of the biomolecules labeled in the step (c), and (e) selecting the keratinocytes in which the signal intensity of at least three biomolecules labeled in the step (b) is larger than the signal intensity of the biomolecules labeled in the step (c). An independent claim is also included for kit-of-parts comprising (A) the reference sample which is selected such that it is suitable for producing the three-dimensional epidermal models for toxicological in vitro tests for determining the skin irritation, (B) antibodies which are specific against at least three different biomolecules, and (C) optionally at least one substrate which is reacted by the enzyme bonded to the antibody.
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