首页> 外国专利> A method of identifying a cell having an intracellular concentration of a particular metabolite which is higher than its wild type, wherein the alteration of the cell is achieved by recombining, and a method of producing a genetically modified cell of its wild type with optimized production of a particular metabolite, a method of Production of this metabolite, as well as suitable nucleic acids

A method of identifying a cell having an intracellular concentration of a particular metabolite which is higher than its wild type, wherein the alteration of the cell is achieved by recombining, and a method of producing a genetically modified cell of its wild type with optimized production of a particular metabolite, a method of Production of this metabolite, as well as suitable nucleic acids

机译：一种鉴定细胞内特定代谢物浓度高于其野生型浓度的细胞的方法，其中所述细胞的改变是通过重组来实现的，以及一种生产野生型基因修饰的细胞并优化其产量的方法。特定的代谢产物，该代谢产物的生产方法以及合适的核酸

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摘要

The invention relates to a method for the identification of a cell with an intracellular concentration of a certain metabolite, which is increased compared to its wild type, whereby the cell is modified by recombineering, and a method for the production of a genetically modified production cell with optimized production of a specific metabolite Process for the preparation of this metabolite, as well as suitable nucleic acids. According to the invention, a gene coding for a recombimase, which is homologous to a known recombinase gene, is transformed into a cell via a vector and this cell contains a DNA which contains at least one altered gene G1 to Gn or at least one mutation M1 to Mn into the cell and metabolite sensors identify the cell which has the highest metabolite production. From this cell, the mutation responsible for the increased production is isolated, the gene or mutation is removed and introduced into a production strain which thereby exhibits an increased production of the metabolite.

机译：本发明涉及一种用于鉴定具有细胞内某种代谢物浓度的细胞的方法，该方法与野生型相比具有更高的代谢率，从而通过重组来修饰细胞，以及生产基因修饰的生产细胞的方法优化生产特定代谢物的方法制备该代谢物的方法以及合适的核酸。根据本发明，将与已知的重组酶基因同源的编码重组酶的基因通过载体转化到细胞中，并且该细胞包含DNA，该DNA包含至少一个从G1到Gn的改变的基因或至少一个突变。 M1至Mn进入细胞，并由代谢物传感器识别出代谢产物产量最高的细胞。从该细胞中，分离出负责增加产量的突变，去除基因或突变并将其引入到生产菌株中，从而显示出代谢产物的产量增加。

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公开/公告号DE102012024435A1

专利类型
公开/公告日2014-07-10

原文格式PDF
申请/专利权人 FORSCHUNGSZENTRUM JÜLICH GMBH;
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申请/专利号DE102012024435A1
发明设计人 STEPHAN BINDER;LOTHAR EGGELING;MICHAEL BOTT;
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申请日2012-12-14
分类号C12N15/31;
国家 DE
入库时间 2022-08-21 15:37:50

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