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METHOD FOR ASSESSING ARTIFICIAL LIGHT EXPOSURE ON FUNCTIONS OF PERIPHERAL BLOOD NEUTROPHIL GRANULOCYTES

机译:外周血中性粒细胞功能的人工光照评估方法

摘要

FIELD: medicine.;SUBSTANCE: invention refers to medicine, namely to immunology, and can be used to assess the exposure of artificial light on the innate immune factors. That is ensured by assessing the exposure of light generated by light-emitting diodes, incandescent lamps on the neutrophil granulocytes recovered from a donor's peripheral blood. The neutrophil granulocytes are in vitro exposed to light in the light temperature range of 4000 K with solar radiation of 0.03 W/m2 at a wave length of 320 to 400 nm for various periods of time intervals. That is combined with stating the presence or absence of the following variations, such as phagocytic activity increase, lysosomal activity increase, biocidal abilities decrease recorded for the neutropils in the nitro blue tetrazolium reduction test.;EFFECT: using the given method enables assessing the biological safety of the new artificial light sources integrated into an individual's light-colour living environment with using a cell culture of the innate immunity and avoiding direct human participation.;3 tbl
机译:技术领域本发明涉及医学,即免疫学,可用于评估人造光对先天免疫因子的暴露。通过评估由发光二极管,白炽灯产生的光在从供体外周血中回收的嗜中性粒细胞中的暴露程度,可以确保这一点。在4000 K的光温度范围内,以0.03 W / m 2 的太阳辐射,在320至400 nm的波长下,将嗜中性粒细胞在不同的时间段内暴露于光下。结合说明是否存在以下变异,例如吞噬细胞活性增加,溶酶体活性增加,在硝基蓝四唑还原试验中嗜中性粒细胞记录的杀生物能力降低;效果:使用给定方法可以评估生物学通过使用具有先天免疫力的细胞培养物并避免人类直接参与,将新的人工光源的安全性集成到个人的浅色生活环境中。3tbl

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