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METHOD OF ASSESSMENT OF PROTEIN-SYNTHESIZING FUNCTION OF LEUKOCYTES FOR CLINICAL STUDIES
METHOD OF ASSESSMENT OF PROTEIN-SYNTHESIZING FUNCTION OF LEUKOCYTES FOR CLINICAL STUDIES
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机译:用于临床研究的白细胞蛋白质合成功能评估方法
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摘要
FIELD: biotechnology.;SUBSTANCE: invention describes the method of assessment of protein-synthesizing function of leukocytes for clinical studies with enzyme-immunoassay method by isolation of leukocytes from the venous blood using the system Vacutainer BD, containing dextrose, the procedure of washing cells, preparing a suspension containing 50000 cells in 1 mcm, the subsequent 20-hour cultivation in Needle-MEM medium with addition of tumour necrosis factor-alpha at a concentration of 0.005 mcg/ml, destruction of cell membranes by lysis solution and the 50-fold dilution of the samples in determining the concentration of alpha-defensins, and in determining the content of C-reactive protein without dilution.;EFFECT: improving accuracy and performance of the method.;1 tbl, 3 ex
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机译:领域:本发明描述了用于白细胞的蛋白合成功能的评估方法,该方法用于通过酶免疫测定法,通过使用含有葡萄糖的Vacutainer BD系统从静脉血中分离白细胞,洗涤细胞的程序,进行酶免疫测定法,准备在1 mcm的细胞中含有50000个细胞的悬浮液,然后在Needle-MEM培养基中培养20小时,并添加浓度为0.005 mcg / ml的肿瘤坏死因子-α,用裂解液破坏细胞膜,并在50-将样品稀释两倍以测定α-防御素的浓度,并确定未经稀释的C反应蛋白的含量。效果:提高方法的准确性和性能。1 tbl,3 ex
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