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METHOD OF CARRYING OUT PCR-RFLP FOR GENOTYPING CATTLE ON ALLELES A AND K OF GENE DGAT1

机译:在DGAT1基因A和K等位基因上进行牛基因分型的PCR-RFLP方法

摘要

FIELD: veterinary medicine.;SUBSTANCE: method of carrying out PCR-RFLP for genotyping cattle on alleles A and K of gene DGAT1, which is different from the nearest prototype [1] in that at the stage of PCR the other sequences of the oligonucleotide primers: DGAT1-1: 5'- CCGCTTGCTCGTAGCTTTCGAAGGTAACGC-3' (SEQ ID NO:1): DGAT1-2: 5'-CCGCTTGCTCGTAGCTTTGGCAGGTAACAA-3' (SEQ ID NO:2): DGAT1-3: 5'-AGGATCCTCACCGCGGTAGGTCAGG-3' (SEQ ID NO:3) are used, and at the stage of RFLP the other restriction endonuclease - TaqI is used, with the generation of the genotype-specific fragments: the genotype AA=82/18 bp, the genotype KK=100 bp and the genotype AA=100/82/18 bp (Figures 1 and 2).;EFFECT: development of an efficient method of genotyping the cattle on DGAT1-gene based on PCR-RFLP analysis.;2 dwg, 2 tbl
机译:领域:兽医:对牛DGAT1基因A和K等位基因进行基因分型的PCR-RFLP方法,与最近的原型[1]不同的是,在PCR阶段,寡核苷酸的其他序列引物:DGAT1-1:5'- CCGCTTGCTCGTAGCTTTCGAAGGTAACGC-3'(SEQ ID NO:1):DGAT1-2:5'-CCGCTTGCTCGTAGCTTTGGCAGGTAACAA-3'(SEQ ID NO:2):DGAT1-3:5'-AGGATCCTCACCGCGGTAGGTCAGG-3 (SEQ ID NO:3),并在RFLP阶段使用其他限制性核酸内切酶-TaqI,并产生基因型特异性片段:基因型AA = 82/18 bp,基因型KK = 100 bp和基因型AA = 100/82/18 bp(图1和2)。效果:开发了一种基于PCR-RFLP分析对DGAT1基因进行牛基因分型的有效方法。2dwg,2 tbl

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