首页> 外国专利> METHOD OF DIFFERENTIATING BACILLUS ANTHRACIS FROM OTHER CLOSELY RELATED SPECIES OF GENUS BACILLUS BASED ON DETERMINING DIFFERENCES IN STRUCTURE OF CHROMOSOMAL GENES

METHOD OF DIFFERENTIATING BACILLUS ANTHRACIS FROM OTHER CLOSELY RELATED SPECIES OF GENUS BACILLUS BASED ON DETERMINING DIFFERENCES IN STRUCTURE OF CHROMOSOMAL GENES

机译:确定染色体基因结构差异的方法,将芽孢杆菌与其他近缘芽孢杆菌属区分开

摘要

FIELD: biotechnology.;SUBSTANCE: method includes sample preparation, DNA isolation, PCR statement. At that in carrying out PCR, the oligonucleotide primers are used, which are complementary to sequences of the chromosomal genes fliC and hom2, having the following sequences: fliC-F: 5'-TGGAGCAGTAACAATTGG-3', fliC-R: 5'-GCACCACTGATAGAAATGTTAG-3', hom2-F: 5'-GACGTGTTAAAAGAAGCCCA-3', hom2-R: 5'-CACCAATTTCGTCTTTTACA-3', followed by electrophoretic analysis of the amplification products, when the formation of the amplification product is 153 bps in size it is indicative of belonging of the strain under study to the species B.anthracis, formation of the amplification product with size of 550 bps is indicative of belonging of the strain under study to the other species of the genus Bacillus.;EFFECT: invention enables to differentiate effectively the bacillus anthracis from other species of bacilli.;1 dwg, 1 ex
机译:领域:生物技术;实体:方法包括样品制备,DNA分离,PCR陈述。在进行PCR时,使用寡核苷酸引物,其与染色体基因fliC和hom2的序列互补,具有以下序列:fliC-F:5'-TGGAGCAGTAACAATTGG-3',fliC-R:5'- GCACCACTGATAGAAATGTTAG-3',hom2-F:5'-GACGTGTTAAAAGAAGCCCA-3',hom2-R:5'-CACCAATTTCGTCTTTTACA-3',然后在扩增产物形成大小为153 bps时进行电泳分析它表明所研究的菌株属于炭疽芽孢杆菌种,大小为550 bps的扩增产物的形成表明所研究的菌株属于芽孢杆菌属的其他种。以便有效地将炭疽杆菌与其他细菌区分开来; 1 dwg,1 ex

著录项

相似文献

  • 专利
  • 外文文献
  • 中文文献
获取专利

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号