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WAY DIFFERENTIAL DIAGNOSIS Cervical Dysplasia AND CERVICAL CANCER

机译:鉴别诊断宫颈不典型增生和宫颈癌

摘要

A method for differential diagnosis of cervical dysplasias and cervical cancer based on determining the relative abundance of mRNA of the gene c-fos using quantitative PCR, which involves sampling of the material by biopsy of the uterine cervix, the allocation of the total RNA sample tissue, the preparation of cDNA and amplifying mRNA gene c- fos, characterized in that the presence of cervical cytology dysplasia analyze mRNA expression gene TATA binding protein (TBP), and then compared the level of mRNA expression r ene c-fos mRNA expression level of TBP and if the ratio of mRNA expression levels of c-fos mRNA TBP over 109 RLU - is diagnosed with severe dysplasia, if the value of this ratio from 18 to 35 RLU - diagnosed preinvasive cervical cancer (carcinoma in situ), if the value of the ratio is greater than 148 RLU - diagnosed with squamous microinvasive cervical cancer, if the value of the ratio is greater than 202 RLU - diagnosed with squamous invasive cervical cancer.
机译:一种通过定量PCR确定c-fos基因mRNA相对丰度的宫颈异型增生和宫颈癌的鉴别诊断方法,该方法涉及通过宫颈活检对材料进行采样,对总RNA样品组织进行分配,制备cDNA并扩增mRNA基因c-fos,其特征是在宫颈细胞学异常增生的存在下分析mRNA表达基因TATA结合蛋白(TBP),然后比较mRNA表达水平和c-fosmRNA表达水平。 TBP,如果c-fos mRNA TBP的mRNA表达水平超过109 RLU的比率被诊断为严重不典型增生,如果该比率的值在18至35 RLU之间,则被诊断为浸润性宫颈癌(原位癌),如果该值如果该比值的值大于202 RLU-诊断为鳞状浸润性宫颈癌,则该比值大于148 RLU-诊断为鳞状微浸润宫颈癌。

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