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首页> 外国专利> RECOMBINANT PLASMID DNA pPA-OPRF-ETA, WHICH CODES SYNTHESIS OF HYBRID RECOMBINANT PROTEIN, WHICH INCLUDES AMINO-ACID SEQUENCES OF PROTEIN F OF OUTER MEMBRANE AND EXOTOXIN A PSEUDOMONAS aeruginosa, DEFORMATION OF ESCHERICHIA coli PA-OPRF-ETA - PRODUCER OF HYBRID RECOMBINANT PROTEIN, WHICH INCLUDES AMINO-ACID SEQUENCES OF PROTEIN F OF OUTER MEMBRANE AND EXOTOXIN A PSEUDOMONAS aeruginosa, AND METHOD FOR PRODUCTION OF HYBRID RECOMBINANT PROTEIN, WHICH INCLUDES AMINO-ACID SEQUENCES OF PROTEIN F OF OUTER MEMBRANE AND EXOTOXIN A PSEUDOMONAS aeruginosa

RECOMBINANT PLASMID DNA pPA-OPRF-ETA, WHICH CODES SYNTHESIS OF HYBRID RECOMBINANT PROTEIN, WHICH INCLUDES AMINO-ACID SEQUENCES OF PROTEIN F OF OUTER MEMBRANE AND EXOTOXIN A PSEUDOMONAS aeruginosa, DEFORMATION OF ESCHERICHIA coli PA-OPRF-ETA - PRODUCER OF HYBRID RECOMBINANT PROTEIN, WHICH INCLUDES AMINO-ACID SEQUENCES OF PROTEIN F OF OUTER MEMBRANE AND EXOTOXIN A PSEUDOMONAS aeruginosa, AND METHOD FOR PRODUCTION OF HYBRID RECOMBINANT PROTEIN, WHICH INCLUDES AMINO-ACID SEQUENCES OF PROTEIN F OF OUTER MEMBRANE AND EXOTOXIN A PSEUDOMONAS aeruginosa

机译：重组质体DNA pPA-OPRF-ETA，可合成杂种重组蛋白，包括外膜蛋白F的氨基酸序列和铜绿假单胞菌的外毒素，重组大肠杆菌-胰蛋白酶原-蛋白酶。 ，包括外膜蛋白F和外膜毒素铜绿假单胞菌的氨基酸序列，以及生产杂交重组蛋白的方法，其中包括外膜蛋白F和蛋白质膜外膜蛋白的氨基酸序列

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FIELD: medicine, pharmaceutics.;SUBSTANCE: group of inventions refers to biotechnology. The recombinant plasmid DNA pPA-OPRF-ETA having a size of 8007 base pairs codes the synthesis of recombinant protein OprF-ETA Pseudomonas aeruginosa. The plasmid contains the following fragments: Xba I-Hind III DNA fragment having a size 177 base pairs; Hind III-Hind III DNA fragment having a size of 1059 base pairs; Hind III-Xho I DNA fragment having a size of 1598 base pairs; Sph I-Hind III DNA fragment having a size of 425 base pairs; EcoR I - BamH I DNA fragment having a size of 57 base pairs. There are also presented the bacterial strain Escherichia coli PA-OPRF-ETA producing protein OprF-ETA and prepared by transforming the parent bacterial strain Escherichia coli BL21(DE3) of plasmid DNA pPA-OPRF-ETA and a method for preparing the above protein OprF-ETA.;EFFECT: group of inventions enables preparing the target product weighing approximately 100 kDa.;3 cl, 3 dwg, 1 tbl, 4 ex
机译：领域：医药，药物;物质：一组发明涉及生物技术。具有8007个碱基对的大小的重组质粒DNA pPA-OPRF-ETA编码重组蛋白OprF-ETA铜绿假单胞菌的合成。该质粒包含以下片段：Xba I-Hind III DNA片段，具有177个碱基对的大小;具有1059个碱基对大小的Hind III-Hind III DNA片段;具有1598个碱基对大小的Hind III-Xho I DNA片段;具有425个碱基对大小的Sph I-Hind III DNA片段; EcoR I-BamHI I DNA片段，大小为57个碱基对。还提出了产生细菌菌株大肠杆菌PA-OPRF-ETA的蛋白OprF-ETA，并通过转化质粒DNA pPA-OPRF-ETA的亲本细菌菌株BL21（DE3）来制备，以及制备上述蛋白OprF的方法。 -ETA .;效果：一组发明使得能够制备重量约为100 kDa的目标产品; 3 cl，3 dwg，1 tbl，4 ex

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