首页> 外国专利> SNP primer for discrimination of Chunpoong cultivar of Panax ginseng C.A. Meyer and method for discriminating the Chunpoong cultivar using the same

SNP primer for discrimination of Chunpoong cultivar of Panax ginseng C.A. Meyer and method for discriminating the Chunpoong cultivar using the same

机译:SNP引物用于人参C.A.春蓬品种的鉴别。迈耶和使用该方法鉴别春蓬品种的方法

摘要

PURPOSE: A single nucleotide polymorphism (SNP) primer for determining the variety of Panax ginseng C.A. Meyer and a primer set by the combination thereof are provided to effectively distinguish Chunpoong from different varieties of Panax ginseng C.A. Meyer through PCR analysis, to effectively select breeding resources of the new variety, and to prevent entrance of different varieties of Panax ginseng C.A. Meyer. CONSTITUTION: A gene of Auxin repressed protein for determining the variety of Panax ginseng C.A. Meyer is selected among sequences of sequence numbers 1-10. An SNP primer for determining the variety of Panax ginseng C.A. Meyer specifically amplifies the gene of auxin repressed protein of sequence number 1. The SNP primer does not specifically amplify one or more genes of auxin repressed protein of sequence numbers 2-10. A method for determining the variety of Panax ginseng C.A. Meyer comprises the steps of: extracting DNA from a ginseng sample; performing PCR using the SNP primer and preparing a PCR product; and analyzing the length of the PCR product. [Reference numerals] (AA) Positive marker; (BB) Negative marker
机译:目的:用于确定人参C.A.品种的单核苷酸多态性(SNP)引物。提供Meyer及其组合引物,可有效区分春蓬和人参C.A.的不同品种。迈耶通过PCR分析,有效地选择了新品种的育种资源,并防止人参C.A.的不同品种进入。迈耶组成:生长素抑制蛋白的一个基因,用于确定人参C.A.的品种。 Meyer是在序列号1-10的序列中选择的。用于确定人参C.A.品种的SNP引物Meyer特异性扩增了序号为1的生长素抑制蛋白的基因。SNP引物未特异性扩增序号为2-10的生长素抑制蛋白的一个或多个基因。一种确定人参C.A.品种的方法Meyer包括以下步骤:从人参样品中提取DNA;使用所述SNP引物进行PCR并制备PCR产物;并分析PCR产物的长度。 [参考数字](AA)正标记; (BB)负标记

著录项

  • 公开/公告号KR101375952B1

    专利类型

  • 公开/公告日2014-03-18

    原文格式PDF

  • 申请/专利权人

    申请/专利号KR20120013115

  • 申请日2012-02-09

  • 分类号C12N15/29;C12Q1/68;

  • 国家 KR

  • 入库时间 2022-08-21 15:41:18

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