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Recombinant virus-induced gene silencing vector from SYMMV useful for functional analysis of useful genes in soybean and uses thereof

机译:来自SYMMV的重组病毒诱导的基因沉默载体,用于大豆中有用基因的功能分析及其用途

摘要

invention promoter and full-length (full length) SYMMV nucleic acid sequence to contain , and the full-length sequence of SYMMV recombinant coat protein coding SYMMV origin comprising restriction sites for inserting foreign genes into the gene VIGS (Virus-induced gene silencing) vector , the recombinant VIGS vector in PDS (phytoene desaturase) gene is inserted into a recombinant VIGS vector , the method using the recombinant VIGS vector for analyzing the function of a foreign gene , a method of producing a desired gene is silenced plant using the recombinant VIGS vector , the target gene is silenced plant produced by the method and their seeds , and relates to the primer combinations to amplify the recombinant vector VIGS . ;
机译:本发明的启动子和包含的全长(全长)SYMMV核酸序列,以及编码SYMMV起点的SYMMV重组外壳蛋白的全长序列,该序列包含将外源基因插入基因VIGS(病毒诱导的基因沉默)载体的限制性位点将PDS(八氢番茄红素去饱和酶)基因中的重组VIGS载体插入重组VIGS载体中,使用该重组VIGS载体分析外源基因的功能的方法,使用该重组VIGS沉默植物产生所需基因的方法该靶基因是该方法产生的沉默植物及其种子,并与引物组合有关,以扩增重组载体VIGS。 ;

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