A METHOD FOR PRODUCING POLY HYDROXYBUTYRATE-CO-HYDROXYVALERATE WITH HIGH 3-HYDROXY VALERATE CONTENT USING PROPIONYL-COA TRANSFERASE GENE FROM RALSTONIA EUTROPHA

• 1. 胶原改性聚(3-羟基丁酸酯-3-羟基戊酸酯)及其制备方法与应用 [P] . 中国专利： CN101012306B . 2010.08.11
• 2. 胶原改性聚(3－羟基丁酸酯－3－羟基戊酸酯)及其制备方法与应用 [P] . 中国专利： CN101012306A . 2007-08-08
• 3. A Method for producing poly hydroxybutyratecohydroxyvalerate with High 3-hydroxy valerate Content using propionylcoA transferase gene from Ralstonia eutropha [P] . 韩国专利： KR101518040B1 . 2015-05-18

  机译： 利用富营养小球藻的丙酰辅酶A转移酶基因生产3-羟基戊酸酯含量高的聚羟基丁酸酯/羟基戊酸酯的方法

• 4. New ralstonia eutropha glycosyl-transferase [P] . 德国专利： DE19717893A1 . 1999-01-14

  机译： 新拉氏菌富营养糖基转移酶

• 5. Recombinant Ralstonia eutropha capable of producing polylactic acid or polylatic acid polymer, and method for producing polylactic acid or polylatic acid copolymer using same [P] . 美国专利： US9920338B2 . 2018-03-20

  机译： 能够生产聚乳酸或聚乳酸聚合物的重组富营养小球藻，以及使用它们的生产聚乳酸或聚乳酸共聚物的方法

• 1. Optimization of Propionic Acid Feeding for Prouction of Poly（3—hydroxybutyrate—co—3—hydroxyvalerate）in Fed—Batch Culture of Ralstonia eutropha [J] . LonganSHANG, JinHwanDO, 范代娣, 中国化学工程学报：英文版 . 2003,第002期
• 2. An Integrated Quantitative Trait Locus Map of Oil Content in Soybean, Glycine max(L.)Merr., Generated Using a Meta-Analysis Method for Mining Genes [J] . QI Zhao-ming, CHEN Qing-shan, HAN Xue, 农业科学学报（英文版） . 2011,第011期
• 3. Integration of association and computational methods reveals functional variants of LEPR gene for abdominal fat content in chickens [J] . LI Yu-dong, WANG Wei-jia, LI Zi-wei, 农业科学学报（英文版） . 2021,第010期
• 4. Integration of association and computational methods reveals functional variants of LEPR gene for abdominal fat content in chickens [J] . LI Yu-dong, WANG Wei-jia, LI Zi-wei, 农业科学学报：英文版 . 2021,第010期
• 5. Sequential feeding of glucose and valerate in a fed-batch culture of Ralstonia eutropha for production of poly(hydroxybutyrate-co-hydroxyvalerate) with high 3-hydroxyvalerate fraction [J] . Shang LG, Yim SC, Park HG, Biotechnology Progress . 2004,第1期
• 6. Synthesis and characterization of poly(3-hydroxybutyrate) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) polymer mixtures produced in high-density fed-batch cultures of Ralstonia eutropha (Alcaligenes eutrophus) [J] . Madden LA., Asrar J., Anderson AJ. Macromolecules . 1998,第17期
• 7. Molecular characterization of the poly(3-hydroxybutyrate) (PHB) synthase from Ralstonia eutropha: in vitro evolution, site-specific synthase from Ralstonia eutropha: in vitro evolution, site-specific mutagenesis and development of a PHB synthase prot [J] . Bernd H. A. Rehm, Regina V. Antonio, Patricia Spiekermann, Biochimica et Biophysica Acta. Protein Structure and Molecular Enzymology . 2002,第1期
• 8. Thermal and Rheological Behavior of Binary Blends of Poly(hydroxybutyrate-co-hydroxyvalerate) and Poly(ethylene-co-vinyl acetate) with Different Vinyl Acetate Content [C] . Osvaldo F. Souza Junior, Lucas H. Staffa, Lidiane C. Costa Brazilian Polymer Conference . 2019
• 9. Bioconversion of nonylphenol ethoxylate to poly-(3-hydroxybutyrate) by Ralstonia eutropha and other PHA-producing bacteria. [D] . Blaylock, Morris. 2002
• 10. Genetically Modified Strains of Ralstonia eutropha H16 with β-Ketothiolase Gene Deletions for Production of Copolyesters with Defined 3-Hydroxyvaleric Acid Contents [O] . Nicole Lindenkamp, Elena Volodina, Alexander Steinbüchel 2012
• 11. Ralstonia eutropha H16 Encodes Two and Possibly Three Intracellular Poly[d-(−)-3-Hydroxybutyrate] Depolymerase Genes [O] . York, Gregory M., Lupberger, Joachim, Tian, Jiamin, 2003

• 1. BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung injury.METHODS： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the infection.Their lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi cant.RESULTS： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004） （P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after infection.Compared to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） after infection （P〈0.05）. Compared to group A, pathological changesat 12 hours in group B indicate marked pulmonary vascular congestion, interstitial edema andinfl ammatory infi ltration. Alveolar cavity collapse and boundaries of the alveolar septum could not beclearly identifi ed.CONCLUSION： Vibrio vulnifi cus sepsis can lead to injury in rat lungs, and increased HMGB1expression in lung tissue may be one of the mechanisms for injury from Vibrio vulnifi cus sepsis. [J] . Qiao-meng Qiu ,Zhong-wang Li ,Lu-ming Tang . 世界急诊医学杂志(英文) . 2011,第4期
• 2. 聚羟基脂肪酸酯可降解生物医用材料-Ⅱ:聚-3-羟基丁酸酯共聚物的制备与表征 [C] . 刘炼 ,魏志勇 ,杨璠 . 2005年中国机械工程学会年会 . 2005
• 3. 纳米羟基磷灰石的制备和聚（3-羟基丁酸酯-co-3-羟基戊酸酯）的改性及生物性能研究 [A] . 罗晓刚 . 2007