首页> 外国专利> METHOD FOR ISOLATING NUCLEIC ACID USING MAGNETITE PARTICLE COATED CARBON NANOTUBES

METHOD FOR ISOLATING NUCLEIC ACID USING MAGNETITE PARTICLE COATED CARBON NANOTUBES

机译:用磁铁矿包覆碳纳米管分离核酸的方法

摘要

The present invention discloses a method for isolating nucleic acid using magnetite particle coated carbon nanotubes capable of reducing time for isolating nucleic acid and improving recovery rate by using the principle of positive charge-negative charge coupling. To this end, the method for isolating nucleic acid using magnetite particle coated carbon nanotubes comprises: a step of mixing a sample including nucleic acid with a CNT dispersing liquid containing magnetite particle coated carbon nanotubes and making the mixture react; a step of applying magnetic field to the mixture of the sample and CNT dispersing liquid to separate a nucleic acid conjugate of the nucleic acids and the magnetite particle coated carbon nanotubes; and a step of recovering nucleic acid by adding a DNA eluting reagent to the the conjugate. According to the present invention, as phenol is not used in the method different from a conventional method for isolating with an organic solvent, the method is not harmful for a human body, minimizes the problems such as environmental pollution, and presents binding properties to target DNA and high yield rate, thereby securing sample DNA per unit time in bulk.;COPYRIGHT KIPO 2014
机译:本发明公开了一种使用磁铁矿颗粒包覆的碳纳米管分离核酸的方法,该方法可以通过利用正电荷-负电荷偶联的原理减少分离核酸的时间并提高回收率。为此,使用磁铁矿颗粒被覆的碳纳米管分离核酸的方法包括以下步骤:将包含核酸的样品与含有磁铁矿颗粒被覆的碳纳米管的CNT分散液混合,并使混合物反应。对样品和CNT分散液的混合物施加磁场以分离核酸的核酸结合物和磁铁矿颗粒涂覆的碳纳米管的步骤;通过向结合物中添加DNA洗脱试剂来回收核酸的步骤。根据本发明,由于与传统的用有机溶剂分离的方法不同,没有使用苯酚,因此该方法对人体无害,使环境污染等问题最小化,并具有与靶标的结合特性。 DNA和高产率,从而确保每单位时间批量采集样品DNA。; COPYRIGHT KIPO 2014

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