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EXPRESSION AND PURIFICATION METHOD IN ESCHERICHIA COLI OF BIOLOGICALLY ACTIVE HUMAN LIF RECOMBINANT PROTEIN USING HUMAN PDIBA TAG
EXPRESSION AND PURIFICATION METHOD IN ESCHERICHIA COLI OF BIOLOGICALLY ACTIVE HUMAN LIF RECOMBINANT PROTEIN USING HUMAN PDIBA TAG
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机译：人PDIBA标签在生物活性人LIF重组蛋白大肠杆菌中的表达和纯化方法。
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摘要
The present invention relates to an expression and purification method in Escherichia coli of a biologically active human LIF recombinant protein using human PDIba tag, and provides one among a gene coding human protein disulfide isomerase (hPDI) or hPDIba domain gene; a recombinant expression vector comprising human leukemia inhibitory factor (hLIF); a recombinant microorganism transformed to the recombinant expression vector; a manufacturing method of the hLIF recombinant protein (rhLIF); and the hLIF recombinant protein (rhLIF) produced by the manufacturing method.;COPYRIGHT KIPO 2014
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