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Primer and probe for use in detection of mycobacterium kansasii and method for detection of mycobacterium kansasii using the same
Primer and probe for use in detection of mycobacterium kansasii and method for detection of mycobacterium kansasii using the same
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机译：用于检测堪萨斯分枝杆菌的引物和探针以及使用它们的检测堪萨斯分枝杆菌的方法
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摘要
The present invention discloses an oligonucleotide which comprises a part or the entire sequence of the nucleotide sequence depicted in SEQ ID NO: 1, 2, 3 or 4, or a part or the entire sequence of a nucleotide sequence complementary to the nucleotide sequence, wherein the oligonucleotide is capable of hybridizing with the nucleotide sequence of Mycobacterium kansasii; a primer and a probe for use in the detection of Mycobacterium kansasii comprising the oligonucleotide; and a method for detecting Mycobacterium kansasii using the primer and/or probe. The method for detecting Mycobacterium kansasii enables the detection of M . kansasii more rapidly and with higher accuracy compared with a conventional bacterium identification method performed by culture examination on a bacterium. Further, the method can exclude any false positive result for the diagnosis and can also detect and diagnose M. kansasii with higher accuracy compared with a diagnosis method performed by PCR using a conventional primer and/or probe. Still further, the method can quantify a M. kansasii cell.
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机译：本发明公开了一种寡核苷酸，其包含SEQ ID NO：1、2、3或4所示核苷酸序列的部分或全部序列，或与核苷酸序列互补的核苷酸序列的部分或全部序列，其中该寡核苷酸能够与堪萨斯分枝杆菌的核苷酸序列杂交。用于检测堪萨斯分枝杆菌的引物和包含该寡核苷酸的探针;以及使用引物和/或探针检测堪萨斯分枝杆菌的方法。用于检测堪萨斯分枝杆菌的方法能够检测M。与通过对细菌进行培养检查而进行的常规细菌鉴定方法相比，堪萨斯州更快速，更准确。此外，与使用常规引物和/或探针通过PCR进行的诊断方法相比，该方法可以排除用于诊断的任何假阳性结果，并且还可以以更高的准确性检测和诊断堪萨斯分枝杆菌。更进一步，该方法可以定量堪萨斯分枝杆菌细胞。
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