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Method for nucleic acid amplification using a microchip PCR with integrated real-time CE detection
Method for nucleic acid amplification using a microchip PCR with integrated real-time CE detection
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机译:使用具有集成实时CE检测的微芯片PCR进行核酸扩增的方法
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摘要
A microfluidic device is provided with appropriate integrated structures to conduct large volume PCR and end-point or real-time capillary electrophoresis detection The microfluidic device includes a substrate having an amplification chamber of a volume of nucleic acid, wells disposed on the substrate, flow channels connecting the wells and the chamber in the substrate to allow for solution flow through the chamber, and one or more separation channels provided in the substrate and connected to the chamber for separating and detecting a fraction of the amplified nucleic acid The chamber, the flow channels, and the one or more separation channels are configured such that the hydrodynamic flow resistance of the chambers and the flow channels combined is at least 10Λ3 times smaller than the hydrodynamic flow resistance in the one or more separation channels The microfluidic device can achieve a very high sensitivity in detection while being highly cost effective
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