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首页> 外国专利> Procedure for the clinical diagnosis of an infectious disease based on the use of quantitative pcr, labeled oligonucleotides of 8 to 9 nucleotides in length and the ung of atlantic cod (gadus morhua). (Machine-translation by Google Translate, not legally binding)

Procedure for the clinical diagnosis of an infectious disease based on the use of quantitative pcr, labeled oligonucleotides of 8 to 9 nucleotides in length and the ung of atlantic cod (gadus morhua). (Machine-translation by Google Translate, not legally binding)

机译：基于定量pcr，长度为8至9个核苷酸的标记寡核苷酸和大西洋鳕（ungus morhua）幼枝的临床诊断程序。 （通过Google翻译进行机器翻译，没有法律约束力）

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摘要

The present invention provides a method, useful for use in a clinical microbiology laboratory, which allows the determination of the presence or absence of a microorganism in a biological sample of a subject with a high degree of reliability using the chain reaction of the polymerase (pcr) performed quantitatively in real time. Said procedure comprises the following steps: To. Carry out a treatment of the reaction mixture that will be used to carry out the polymerase chain reaction (pcr) performed quantitatively in real time with the uracil-dna-glycosylase from the atlantic cod (gadus morhua) of seq id no. 3 or with a variant thereof; y B. Determine the presence or absence of the microorganism in the biological sample, after treatment of the reaction mixture according to section a), through the determination of the presence or absence of amplicons from the polymerase chain reaction (pcr) carried out quantitatively in real time; Wherein said polymerase chain reaction (pcr) employs dna, complementary dna (cdna) or single-stranded dna obtained by retrotranscription of ribonucleic acid (rna) from the biological sample, at least one pair of specific primers, dntps, a buffer of suitable reaction, a thermostable dna polymerase and a fluorescently labeled probe, wherein said fluorescently labeled probe consists of an oligonucleotide with a length of 8 to 9 nucleotides labeled with a fluorophore, preferably said probe has a length of 8 nucleotides. (Machine-translation by Google Translate, not legally binding)
机译：本发明提供了一种可用于临床微生物实验室的方法，该方法允许使用聚合酶（pcr）的链反应以高度的可靠性确定受试者的生物样品中微生物的存在或不存在。 ）实时定量执行。所述过程包括以下步骤：To。对反应混合物进行处理，该反应混合物将与来自序列号为7的大西洋鳕鱼（gadus morhua）的尿嘧啶-dna-糖基化酶实时定量地进行聚合酶链反应（pcr）。 3或其变体; y B.在根据a）节处理反应混合物后，通过确定在聚合酶链反应（pcr）中定量进行的扩增子的存在或不存在，确定生物样品中是否存在微生物。即时的;其中所述聚合酶链反应（pcr）采用dna，互补dna（cdna）或通过从生物样品中核糖核酸（rna）逆转录获得的单链dna，至少一对特异性引物，dntps，合适反应的缓冲液，热稳定的dna聚合酶和荧光标记的探针，其中所述荧光标记的探针由长度为8至9个核苷酸的寡核苷酸组成，所述寡核苷酸被荧光团标记，优选地所述探针的长度为8个核苷酸。 （通过Google翻译进行机器翻译，没有法律约束力）

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