首页> 外国专利> Method for the detection of IL-16 activity and modulation of IL-16 activity based on proxy RANTES levels

Method for the detection of IL-16 activity and modulation of IL-16 activity based on proxy RANTES levels

机译:基于代理RANTES水平的IL-16活性检测和IL-16活性调节的方法

摘要

A method of detecting the biological activity of IL - 16 in a sample comprising the following steps: a) providing a first population of eukaryotic cells surrounding the means and responsive to the biological activity of IL - 16 with a first sample of testing; b) providing a second population of eukaryotic cells surrounding the means and responsive to the biological activity of IL - 16 with a negative control sample; c) measuring the amount of a proxy of RANTES produced by the first and second populations of eukaryotic cells; and d) comparing the amount proxy RANTES produced by the first and second populations of eukaryotic cells, in which a smaller proxy amount of RANTES produced by a first population of eukaryotic cells relative to the level of proxy of RANTES produced by a second eukaryotic cell population indicates the detection of biological activity of IL - 16 in a test sample, wherein the proxy RANTES is a peptide chain with at least 75% identity in amino acid residues 1 to 68 of SEQ ID No. 4 or a nucleic acid encoding a peptide chain with at least 75% identity in amino acid residues 1 to 68 of SEQ ID NO: 4.
机译:一种检测样品中IL-16生物学活性的方法,包括以下步骤:a)用第一份测试样品提供围绕所述装置并且对IL-16生物学活性有反应的第一真核细胞群; b)用阴性对照样品提供围绕所述装置并且对IL-16的生物学活性有响应的第二真核细胞群; c)测量由第一和第二组真核细胞产生的RANTES代理的数量; d)比较第一和第二真核细胞群产生的代理RANTES的量,其中第一真核细胞群体产生的RANTES的替代量相对于第二真核细胞群体产生的RANTES的替代量较小表示检测到测试样品中IL-16的生物学活性,其中代理RANTES是在SEQ ID No. 4的氨基酸残基1至68中具有至少75%的同一性的肽链或编码该肽链的核酸在SEQ ID NO:4的氨基酸残基1至68中具有至少75%的同一性。

著录项

  • 公开/公告号ES2437315T3

    专利类型

  • 公开/公告日2014-01-10

    原文格式PDF

  • 申请/专利号ES20070798159T

  • 发明设计人 GLASS WILLIAM G.;

    申请日2007-06-06

  • 分类号G01N33/68;C07K14/52;G01N33/50;

  • 国家 ES

  • 入库时间 2022-08-21 15:55:16

相似文献

  • 专利
  • 外文文献
  • 中文文献
获取专利

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号