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Method for the detection of IL-16 activity and modulation of IL-16 activity based on proxy RANTES levels
Method for the detection of IL-16 activity and modulation of IL-16 activity based on proxy RANTES levels
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机译:基于代理RANTES水平的IL-16活性检测和IL-16活性调节的方法
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摘要
A method of detecting the biological activity of IL - 16 in a sample comprising the following steps: a) providing a first population of eukaryotic cells surrounding the means and responsive to the biological activity of IL - 16 with a first sample of testing; b) providing a second population of eukaryotic cells surrounding the means and responsive to the biological activity of IL - 16 with a negative control sample; c) measuring the amount of a proxy of RANTES produced by the first and second populations of eukaryotic cells; and d) comparing the amount proxy RANTES produced by the first and second populations of eukaryotic cells, in which a smaller proxy amount of RANTES produced by a first population of eukaryotic cells relative to the level of proxy of RANTES produced by a second eukaryotic cell population indicates the detection of biological activity of IL - 16 in a test sample, wherein the proxy RANTES is a peptide chain with at least 75% identity in amino acid residues 1 to 68 of SEQ ID No. 4 or a nucleic acid encoding a peptide chain with at least 75% identity in amino acid residues 1 to 68 of SEQ ID NO: 4.
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机译:一种检测样品中IL-16生物学活性的方法,包括以下步骤:a)用第一份测试样品提供围绕所述装置并且对IL-16生物学活性有反应的第一真核细胞群; b)用阴性对照样品提供围绕所述装置并且对IL-16的生物学活性有响应的第二真核细胞群; c)测量由第一和第二组真核细胞产生的RANTES代理的数量; d)比较第一和第二真核细胞群产生的代理RANTES的量,其中第一真核细胞群体产生的RANTES的替代量相对于第二真核细胞群体产生的RANTES的替代量较小表示检测到测试样品中IL-16的生物学活性,其中代理RANTES是在SEQ ID No. 4的氨基酸残基1至68中具有至少75%的同一性的肽链或编码该肽链的核酸在SEQ ID NO:4的氨基酸残基1至68中具有至少75%的同一性。
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