首页> 外国专利> METHOD FOR SIMULATING OPEN PENETRATING DOSED CEREBROSPINAL TRAUMA IN EXPERIMENTAL ANIMALS

METHOD FOR SIMULATING OPEN PENETRATING DOSED CEREBROSPINAL TRAUMA IN EXPERIMENTAL ANIMALS

机译:实验动物模拟开放性透皮给药的脊髓损伤的方法

摘要

A method for the simulation of an open penetrating dosed cerebrospinal trauma consists in that a thin needle is introduced into interarch space, puncture of spinal pia mater is carried out and destruction of spinal cord is performed by dosed injection of a heparinized autoblood to spinal cord tissue at the level of the T11-T12 spinal cord segments of the pubertal male rats. General anesthesia is used with mixture of solutions of xylazine (15 mg/kg) and ketamine (70 mg/kg), access to the specified spinal cord segments is formed by perforation of interarch ligament by needle through which autologous heparinized blood obtained from a caudal vein will be injected, immersion of the needle in the tissue of spinal cord before formation of locomotory equivalents in both hind feet and injection of autologous blood in amount of 0.1 ml. the completion of surgical intervention and postoperative care of animals is carried out in the usual manner.
机译:一种模拟开放性穿透性剂量性脑脊髓损伤的方法,其特征在于,将细针穿入足弓间隙,穿刺脊髓半圆形椎体,并通过将肝素化的自体血按剂量注射至脊髓组织来破坏脊髓在青春期雄性大鼠的T11-T12脊髓节段水平全身麻醉与甲苯噻嗪(15 mg / kg)和氯胺酮(70 mg / kg)的混合物混合使用,通过针刺弓状韧带穿孔形成指定的脊髓节段,通过该节段从尾椎获得的肝素自体血将静脉注射,将针浸入脊髓组织中,然后在后足形成运动等同物,并注射0.1 ml的自体血。动物的手术干预和术后护理的完成以通常的方式进行。

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