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BIOELECTRICITY, WASTE REMEDIATION AND INCREASED NITROGENASE ACTIVITY IN PLANTS BY CLONING AND EXPRESSION OF NIFF, NIFJ GENE IN GEOBACTER METALLIREDUCENS
BIOELECTRICITY, WASTE REMEDIATION AND INCREASED NITROGENASE ACTIVITY IN PLANTS BY CLONING AND EXPRESSION OF NIFF, NIFJ GENE IN GEOBACTER METALLIREDUCENS
The present invention provides a recombinant1 transformed G. metallireducens bacterium and a method for production thereof which leads to generation of electricity from waste organic matter and metals. It also produces high content of nitrogenase for use by plants. In the present study, nifF gene from Azotobacter vinelandii DJ and niff gene from Lactococcus lactis subsp. lactis I11403 are cloned in the BarnHI, EcoRI sites respectively of the pEA3 vector. After transformation in G. metallireducens, five out of six constructs are found to be stable. Screening of recombinant clones was done by in situ hybridisation using 32~-labellendi fF, niff DNA as the probe. The mechanism enables nitrogen fixation and its availability to plants at a large scale. Also the surface transport of electrons and the electron transport chain, made it possible to produce bioelectricity. The developed bio-remediating and bio-electricity generating mechanism may be applied for cleaning of metal and organic waste and converting dumping grounds to grow green plants, all at very cheap rates.
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