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Infection clone of wheat dwarfism virus, reaction mixture and primers for detection thereof

机译:小麦矮化病毒的感染克隆,反应混合物及其检测引物

摘要

Solution relates to an infectious clone of wheat dwarf virus, and further reaction and primers for its detection. The peak contains at least one copy of the viral genome of the wheat dwarf virus comprising an intact replication origin sequence contained within the LIR region, wherein the copy is continuously followed by a portion of the second copy, so that the part of the second copy was at least contained intact sequence of the replication origin, respectively. replication end when infectious clone stunt virus wheat together comprising less than two complete copies of the viral stunt virus genome of wheat, said copies are inserted inside sequences commonly used DNA bacterial plasmids serving for DNA replication, selected from the group pUC18 pGWM-T Easy and derivations unchanging their original purpose, ie. mere DNA replication.
机译:溶液涉及小麦矮病毒的传染性克隆,以及进一步的反应和用于检测其的引物。该峰包含至少一个包含在LIR区域内的完整复制起点序列的小麦矮病毒病毒基因组拷贝,其中该拷贝连续跟随第二拷贝的一部分,从而第二拷贝的一部分至少分别含有完整的复制起点序列。当传染性克隆特技病毒小麦一起包含少于两个完整的小麦病毒特技病毒基因组拷贝时,复制结束,所述拷贝插入用于DNA复制的常用DNA细菌质粒的序列内,所述质粒选自pUC18 pGWM-T Easy和衍生品不变其原始目的,即仅DNA复制。

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