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Infection clone of wheat dwarfism virus, reaction mixture and primers for detection thereof
Infection clone of wheat dwarfism virus, reaction mixture and primers for detection thereof
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机译:小麦矮化病毒的感染克隆,反应混合物及其检测引物
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摘要
Solution relates to an infectious clone of wheat dwarf virus, and further reaction and primers for its detection. The peak contains at least one copy of the viral genome of the wheat dwarf virus comprising an intact replication origin sequence contained within the LIR region, wherein the copy is continuously followed by a portion of the second copy, so that the part of the second copy was at least contained intact sequence of the replication origin, respectively. replication end when infectious clone stunt virus wheat together comprising less than two complete copies of the viral stunt virus genome of wheat, said copies are inserted inside sequences commonly used DNA bacterial plasmids serving for DNA replication, selected from the group pUC18 pGWM-T Easy and derivations unchanging their original purpose, ie. mere DNA replication.
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