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L1 reaction mixture for molecular detection of Potato Leaf Roll Virus (PLRV) using quantitative RT-PCR

机译:使用定量RT-PCR进行马铃薯叶卷病毒(PLRV)分子检测的L1反应混合物

摘要

ufeffu0159eu0161enu00ed refers to the reaction mixture for the molecular detection of potato leaf roll virus l1 (plrv) using quantitative rt-pcr, which is characterised by the fact that contains 1.mi.l tested rna sample, reagent containing a mixture of enzymes reverse transcriptase and the dna polymerase mix nucleotide mgcl.sub.2.n. buffer containing magnesium chloridesterile distilled water to a final volume of 25 additional reaction mixture.mi.l and primers specific nucleotide sequence of l - 499f: aag aag tat ccc tca aag cct, l - 675r: tta gcg cgc cct tgt aaa tca agc, amplifikuju00edcu00ed fragment size 176 bp, and the probe l - 567s: tg gac gct tgc gac gag gtt gre ttt with the probe labelling fluorescent dye 6 - carboxyfluores cein (i) and is used as a zhu00e1u0161eu010d tetra - metylkarboxyrhodamin (tamra).
机译:指使用定量rt-pcr分子检测马铃薯叶卷病毒l1(plrv)的反应混合物,其特征在于包含1.mi.l测试的rna样品,试剂含有酶逆转录酶和dna聚合酶混合物核苷酸mgcl.sub.2.n的混合物。含氯化镁无菌蒸馏水的缓冲液至最终体积为25的其他反应混合物。mi.l和引物的特定核苷酸序列为l-499f:aag aag tat ccc tca aag cct,l-675r:tta gcg cgc cct tgt aaa tca agc,扩增片段大小为176 bp的片段,并且探针1-567s:tg gac gct tgc gac gag gtt gre ttt,探针标记荧光染料6-羧基荧光素(i),用作zh u00e1 u016​​1e u010d四-甲氧基核糖泛素(tamra)。

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