首页> 外国专利> GRB2, GATA3, IGM, MAVS and Regula genes were used to determine the expression patterns of infectious anemia and drug resistance in Atlantic salmon salar.

GRB2, GATA3, IGM, MAVS and Regula genes were used to determine the expression patterns of infectious anemia and drug resistance in Atlantic salmon salar.

机译:GRB2,GATA3,IGM,MAVS和Regula基因被用于确定大西洋鲑鱼鳍中传染性贫血的表达模式和耐药性。

摘要

Using genes selected from the group consisting of Grb2, Gata3, IgM, MAVS and Regul genenes that serve as predictors to determine susceptible and resistant phenotypes to infectious salmon anemia (ISA) in Atlantic salmon (Salmo salar), quantifying the profile expression of these genes using PCR analysis in real time, and wherein a sample with the expression pattern for a defiance by cohabitation with ISA, which has no significant difference when compared to the expression pattern of these genes in a control sample without defiance by cohabitation to ISA, is indicative of ISA resistant phenotype, namely, which has as a characteristic, less than 40% mortality for the ISA virus and heritability of said character greater than or equal to 0.35; and a sample having a pattern of expression to a defiance of cohabitation to ISA which happens to be low when compared to a control sample without defiance by cohabitation to ISA, is indicative of a susceptible phenotype to ISA, namely, presented as characteristic , a mortality rate exceeding 50% for the ISA virus and a heritability of such minor or equal to 0.34.
机译:使用选自Grb2,Gata3,IgM,MAVS和Regul基因的基因作为预测因子,以确定对大西洋鲑鱼(Salmo salar)的传染性鲑鱼贫血(ISA)的敏感表型和耐药表型,从而量化这些基因的表达使用实时PCR分析进行分析,其中具有与ISA并存的违抗表达模式的样品是指示性的,与没有与ISA并存的对照样品中这些基因的表达模式相比,该指示性样品具有指示性ISA抗性表型,即具有特征,其对于ISA病毒的死亡率小于40%,并且所述性状的遗传性大于或等于0.35;并且具有相对于没有与ISA共存的抗性的对照样品的表达模式相对于没有与ISA共存的抗性的对照样品而言表现出较低的表达模式的样品指示对ISA的易感表型,即以特征,死亡率表示。 ISA病毒的感染率超过50%,且遗传率较小或等于0.34。

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