首页> 外国专利> TOBACCO PRODUCT, TOBACCO CELL, METHOD FOR REDUCING NORNICOTINE LEVEL ON A PART OF DERIVATIVE PLANT FROM A NICOTIAN GENDER PLANT, METHOD TO REDUCE A POTENTIAL CARCINOGENOUS REDUCINE OF NICOTINE TO NORNICOTIN IN A NICOTIAN PLANT, METHOD FOR IDENTIFYING A GREEN LEAF NICOTINE DEMETILASE SEQUENCE, METHOD FOR IDENTIFYING LOW NORNICOTINE LEVEL TOBACCO PLANT

TOBACCO PRODUCT, TOBACCO CELL, METHOD FOR REDUCING NORNICOTINE LEVEL ON A PART OF DERIVATIVE PLANT FROM A NICOTIAN GENDER PLANT, METHOD TO REDUCE A POTENTIAL CARCINOGENOUS REDUCINE OF NICOTINE TO NORNICOTIN IN A NICOTIAN PLANT, METHOD FOR IDENTIFYING A GREEN LEAF NICOTINE DEMETILASE SEQUENCE, METHOD FOR IDENTIFYING LOW NORNICOTINE LEVEL TOBACCO PLANT

机译：烟草产品，烟草细胞，用于减少源自尼古丁性别的植物的一部分衍生植物上的正烟碱含量的方法，用于将尼古丁植物中烟碱的潜在致癌性还原为诺古丁的方法，鉴定绿色素的方法，用于鉴定低降烟碱水平的烟草植物

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摘要

Compositions and methods for reducing the level of nornicotine and N'-nitrosonomicotine (NNN) in Nicotiana plants and plant parts thereof are provided. The compositions comprise isolated polynucleotides and polypeptides for cytochrome P450s that are involved in the metabolic conversion of nicotine to nornicotine in these plants. Expression cassettes, vectors, plants and plant parts thereof comprising inhibitory sequences that target expression or function of the disclosed cytochrome P450 polypeptides are also provided. Methods for the use of these novel sequences to inhibit expression or function of cytochrome P450 polypeptides involved in this metabolic conversion are also provided. The methods find use in the production of tobacco products that have reduced levels of nornicotine and its carcinogenic metabolite, NNN, and thus reduced carcinogenic potential for individuals consuming these tobacco products or exposed to secondary smoke derived from these products.

机译：提供了降低烟草植物及其植物部分中降烟碱和N'-亚硝基烟碱（NNN）水平的组合物和方法。所述组合物包含用于细胞色素P450的分离的多核苷酸和多肽，所述细胞色素P450与这些植物中烟碱向新烟碱的代谢转化有关。还提供了表达盒，载体，植物及其植物部分，其包含靶向所公开的细胞色素P450多肽的表达或功能的抑制序列。还提供了使用这些新序列来抑制参与该代谢转化的细胞色素P450多肽的表达或功能的方法。该方法可用于生产降烟碱和其致癌代谢物NNN含量降低的烟草产品，从而降低了食用这些烟草产品或暴露于衍生自这些产品的二次烟雾的个人的致癌潜力。

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公开/公告号BRPI0820042A2

专利类型
公开/公告日2014-10-14

原文格式PDF
申请/专利权人 NORTH CAROLINA STATE UNIVERSITY;UNIVERSITY OF KENTUCKY RESEARCH FOUNDATION;
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申请/专利号BR2008PI20042
发明设计人 RALPH E. DEWEY;BALAZS SIMINSZKY;STEVEN W. BOWEN;LILY GAVILANO;
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申请日2008-11-12
分类号C12N15/53;A01H5/12;C12N15/82;C12Q1/68;
国家 BR
入库时间 2022-08-21 15:58:30

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