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Microbial detection apparatus, microbial detection method, and sample container used therein

机译:微生物检测设备,微生物检测方法以及其中使用的样品容器

摘要

The sample container has a two-layer membrane filter comprising a first layer as an upper layer serving as a hydrophilic membrane filter and a hydrophobic membrane filter as an underlying second layer capable of filtering an aqueous solution without the use of a wetting agent and by means of a formed negative pressure. Using this sample container, a large amount of an aqueous sample solution is filtered by means of a negative pressure formed by a suction portion to capture microbes in the aqueous sample solution by the hydrophilic membrane filter. Then, the negative pressure is restored to normal pressure, and a microbial dissolution solution is then added to the membrane filter to retain the microbial dissolution solution for a given time on the hydrophobic membrane filter. Then, the microbial dissolution solution is dispensed to a reaction container containing a luminescent reagent, and luminescence is detected to detect the microbes.
机译:样品容器具有两层膜过滤器,其包括第一层作为上层,用作亲水膜过滤器,而疏水膜过滤器作为下层第二层,其能够在不使用润湿剂的情况下通过以下方式过滤水溶液:形成的负压。使用该样品容器,通过由抽吸部形成的负压来过滤大量的水性样品溶液,以通过亲水膜过滤器捕获水性样品溶液中的微生物。然后,将负压恢复到常压,然后将微生物溶解溶液添加到膜过滤器中,以使微生物溶解溶液在疏水性膜过滤器上保留给定的时间。然后,将微生物溶解溶液分配到装有发光剂的反应容器中,并检测发光以检测微生物。

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