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High-throughput and non-invasive method to vitrify porcine embryos

机译:高通量非侵入性玻璃化猪胚胎的方法

摘要

The present invention provides a practical, non-invasive, and efficient method for cryopreservation of an in-vitro-produced porcine embryo. The inventive method treats the NP (such as IVF- or NT-derived) embryo at the one-cell or cleavage stage prior to compaction with high osmolality followed by high speed centrifugation. The high osmolality treatment enlarges the periviteline space, and with centrifugation, enables the separation of the lipids from the cytoplasm. The lipid-separated embryos after high osmolality treatment have been successfully cryopreserved and later recovered and transferred to produce live offspring.
机译:本发明提供了一种用于冷冻保存体外产生的猪胚胎的实用,无创且有效的方法。本发明的方法在高渗透压紧实然后高速离心之前,在单细胞或分裂阶段处理NP(例如IVF或NT来源的)NP胚胎。高克分子渗透压处理扩大了橄榄石的空间,并通过离心作用,使脂质从细胞质中分离出来。经过高渗透压处理后,脂质分离的胚胎已成功冷冻保存,随后恢复并转移以产生活后代。

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