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Novel Microbial Biocatalysts That Enables Use Of Cellodextrin As Biofuel
Novel Microbial Biocatalysts That Enables Use Of Cellodextrin As Biofuel
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机译：能够使用纤维糊精作为生物燃料的新型微生物生物催化剂
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摘要
The present disclosure provides genetically engineered biocatalysts which enable intracellular assimilation of cellodextrin. The genetically engineered biocatalyst co-expresses a cellodextrin phosphorylase (CDP) gene and a cellobiose phosphorylase (CBP) gene. Further, the genetically engineered biocatalyst includes a first synthetic promoter used to express the cellodextrin phosphorylase (CDP) gene and a second synthetic promoter used to express the cellobiose phosphorylase (CBP) gene. Furthermore, the genetically engineered biocatalyst expresses one or more cellodextrin permeases. The intracellular assimilation includes hydrolysis and phosphorolysis mechanism. Further provided are methods of using the genetically engineered biocatalysts to generate various useful end-products including alcohol and lactic acid.
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