首页>
外国专利>
ISOTOPIC LABELING FOR THE MEASUREMENT OF GLOBAL PROTEIN LEVELS AND TURNOVER IN VIVO
ISOTOPIC LABELING FOR THE MEASUREMENT OF GLOBAL PROTEIN LEVELS AND TURNOVER IN VIVO
展开▼
机译:用于测量体内全球蛋白质水平和营业额的同位素标记
展开▼
页面导航
摘要
著录项
相似文献
摘要
An entire complement or plurality of isotopically labeled amino acids are introduced into the diet of a test subject. Sufficient amounts of the isotopically labeled amino acids are provided to the subject in order to ensure that the subject incorporates a large percentage of isotopically labeled amino acids into newly synthesized proteins. Tissue samples are removed from the subject at different points in time and proteins are extracted and separated so that different proteins of different tissues can be individually analyzed and their amount and pattern of isotopic labeling can be determined. In a preferred embodiment, the methodology can be combined with proteolytic digestion to peptides and analysis by mass spectrometry in order to measure rates of protein turnover in vivo relating to thousands of different proteins.
展开▼