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In decisive method of interaction parameter and the backing surface where the measuring method null ligand of density
In decisive method of interaction parameter and the backing surface where the measuring method null ligand of density
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机译:在决定性的相互作用参数方法和衬里表面的测量方法中无效配体的密度
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摘要
PROBLEM TO BE SOLVED: To provide a method of determining an interaction parameter to precisely measure the concentration of a binding substance contained in a measuring solution without the need for accurately measuring a fixing quantity of ligands to a support, and a method of measuring the concentration of the binding substrate contained in the measuring solution.;SOLUTION: The method of contacting the measuring solution containing the binding substrate able to interact with the ligand to the surface of the support with ligand fixed thereto to determine the interaction parameter of the binding substrate to the ligand in a process that measures the concentration of the binding substance in the measuring solution, based on a frequency, a dielectric constant, or an optical interference fluctuation of the surface of the support, includes the steps of contacting a known calibrating solution to the surface of the support by the concentration of the binding substance able to interact with the ligand contained therein and measuring the frequency, the dielectric constant, or the optical interference fluctuation to determine the interaction parameter of the binding substance, in the former step or the latter step of contacting the measuring solution to the surface of the support.;COPYRIGHT: (C)2010,JPO&INPIT
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