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Plasmid, bacterial wilt, monitoring method, evaluation method and screening method

机译：质粒，青枯病，监测方法，评价方法和筛选方法

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摘要

PPROBLEM TO BE SOLVED: To provide a plasmid reinforcing the expression of a labeling material in Ralstonia solanacearum, the Rastonia solanacearum reinforcing the expression of the labeling material, a method for monitoring various pathogenic bacteria including the Rastonia solanacearum, a method for evaluating sensitivity or resistance to the various pathogenic bacteria including the Rastonia solanacearum, and a method for screening test substances effective to the various pathogenic bacteria including the Rastonia solanacearum. PSOLUTION: This plasmid has a replication module of the genome of a phage RSS1 having an infective capacity to the Rastonia solanacearum and an expression cassette of the labeling substance. The expression cassette has a phage RSB2-originated MCP (major coat protein) promotor region, a gene encoding the labeling material, and a phage RSB2-originated MCP terminator region. The method of monitoring is performed by culturing a plant so as to elongate its root and/or stem along an agar medium surface made inclined, and observing the labeling substance. PCOPYRIGHT: (C)2011,JPO&INPIT

机译：

要解决的问题：为了提供一种在茄果小球藻中增强标签材料表达的质粒，在茄子中增强标签材料表达的Rastonia solanacearum，一种监测包括Rastonia solanacearum在内的各种致病细菌的方法，一种评估方法对包括蓝枯萎病菌的各种病原菌的敏感性或抗性，以及筛选对包括蓝枯萎病菌的各种病原菌有效的测试物质的方法。

解决方案：该质粒具有噬菌体RSS1的基因组的复制模块，该噬菌体具有对蓝枯菌的感染能力和标记物质的表达盒。该表达盒具有起源于噬菌体RSB2的MCP（主要外壳蛋白）启动子区域，编码标记材料的基因和起源于噬菌体RSB2的MCP终止子区域。监测方法是通过培养植物以使其根和/或茎沿着倾斜的琼脂培养基表面伸长并观察标记物质而进行的。

版权：（C）2011，日本特许厅＆INPIT 展开▼

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公开/公告号JP5548919B2

专利类型
公开/公告日2014-07-16

原文格式PDF
申请/专利权人国立大学法人広島大学;和光純薬工業株式会社;
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申请/专利号JP20090192635
发明设计人藤江誠;山田隆;川崎健;小倉俊樹;
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申请日2009-08-21
分类号C12N15/09;C12N1/15;C12Q1/02;
国家 JP
入库时间 2022-08-21 16:16:15

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