首页> 外国专利> Quantitatively determining concentration of biomass formed by culturing in closed bioreactor, comprises e.g. determining constant factor, calculating electrical conductivities or glucose concentrations, and determining biomass concentration

Quantitatively determining concentration of biomass formed by culturing in closed bioreactor, comprises e.g. determining constant factor, calculating electrical conductivities or glucose concentrations, and determining biomass concentration

机译：定量确定通过在密闭生物反应器中培养形成的生物质的浓度，例如包括确定常数因子，计算电导率或葡萄糖浓度，并确定生物质浓度

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Quantitatively determining concentration of biomass, which is formed by culturing in a closed bioreactor, comprises (a) determining a constant factor (alpha ), (b) calculating electrical conductivities (k i) or glucose concentrations, (c) determining the biomass concentration at a time point (t i), (d) multiplying the factor (alpha ) with several differences of electrical conductivity values or glucose concentration values, (d) performing time-weighted averaging with the determined products, (e) and evaluating the respective average value as the biomass concentration. Quantitatively determining concentration of biomass, which is formed by culturing in a closed bioreactor, comprises (a) determining a constant factor (alpha ), which is specific for respective biomass culture, from at least two measured values of electrical conductivities (k aand k b) or glucose concentrations for a cultivation liquid containing the biomass, and corresponding biomass concentrations (X Aand X B) determined after drying, according to formula alpha is equal to (X B-X A)/(k b-k a), (b) calculating electrical conductivities (k i) or glucose concentrations of the biomass concentration measured within the cultivation liquid at different time points in closed bioreactor by multiplying with the difference of the respective electric conductivity difference values (k iminus k i x) or glucose concentration values, with the constant factor (alpha ), as an absolute value, where x is a value between 1 and maximum number of pre-determined electrical conductivities or glucose concentration values, (c) determining the biomass concentration at time point (t i), (d) multiplying the factor (alpha ) with several differences of electrical conductivity values or glucose concentration values that are pre-measured at different time points, (d) performing time-weighted averaging with the determined products, (e) and evaluating the respective average value as the biomass concentration (x i) at the time point (t i).

机译：定量确定通过在密闭生物反应器中培养而形成的生物质浓度，包括（a）确定常数因子（α），（b）计算电导率（ki）或葡萄糖浓度，（c）确定在一定温度下的生物质浓度。时间点（ti），（d）将因子（alpha）与电导率值或葡萄糖浓度值的几个差值相乘，（d）对所确定的乘积执行时间加权平均，（e）并将各自的平均值评估为生物质浓度。定量确定通过在封闭的生物反应器中培养而形成的生物质的浓度，包括：（a）从至少两个电导率测量值（k a和kb）确定特定于各个生物质培养的恒定因子（alpha）。含有生物质的培养液的葡萄糖浓度或葡萄糖浓度，以及干燥后根据公式α确定的相应生物质浓度（X A和XB）等于（X BX A）/（k bk a），（b）计算电导率（ ki）或葡萄糖浓度，将密闭生物反应器在不同时间点在培养液中测量的生物质浓度乘以各个电导率差值（k iminus kix）或葡萄糖浓度值的差，再乘以常数因子（alpha），作为绝对值，其中x是介于1和最大预定电导率或葡萄糖浓度之间的值（c）确定时间点（ti）的生物质浓度，（d）将因子（alpha）乘以在不同时间点预先测得的电导率值或葡萄糖浓度值的几个差异，（d）对确定的产物（e）进行时间加权平均，并在时间点（ti）评估各自的平均值作为生物质浓度（xi）。

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公开/公告号DE102011120268B3

专利类型
公开/公告日2013-02-28

原文格式PDF
申请/专利权人 TECHNISCHE UNIVERSITAET DRESDEN;
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申请/专利号DE201110120268
发明设计人 HAAS CHRISTIANE;STEINGROEWER JULIANE;LENK FELIX;
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申请日2011-11-30
分类号C12M1/34;G01N27/04;G01N33/487;
国家 DE
入库时间 2022-08-21 16:22:06

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