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Coating substrate with monolayer of self-assembling proteins, by providing protein-containing coating solution, contacting surface of substrate with the protein-containing solution, and removing supernatant solution from coated substrate
Coating substrate with monolayer of self-assembling proteins, by providing protein-containing coating solution, contacting surface of substrate with the protein-containing solution, and removing supernatant solution from coated substrate
The method comprises providing a protein-containing coating solution, contacting a surface of the substrate with the protein-containing solution, removing a supernatant solution from the coated substrate, drying the coated substrate, separating protein units aggregated from an aqueous solution of self-assembling proteins for providing the coating solution, and generating monomers or oligomers of the self-assembling proteins by an addition of a solution of ionic surfactants and a salt-containing, alkaline and/or acidic solution to the protein-containing coating solution and then stabilizing. The method comprises providing a protein-containing coating solution, contacting a surface of the substrate with the protein-containing solution, removing a supernatant solution from the coated substrate, drying the coated substrate, separating protein units aggregated from an aqueous solution of self-assembling proteins for providing the coating solution, and generating monomers or oligomers of the self-assembling proteins by an addition of a solution of ionic surfactants, a salt-containing, alkaline and/or acidic solution to the protein-containing coating solution and then stabilizing, where the ionic surfactant is added so that only the surface-active part of each active protein monomer or predominant protein oligomer is enveloped by surfactant particles. Hydrophobins proteins, surface layer proteins and/or recombinant fusion proteins with two domains are used as self-assembling proteins, where the domain is a self-assembling protein domain and a functional domain. Independent claims are included for: (1) a protein-containing coating solution; and (2) a substrate with a protein-containing coating of self-assembling proteins.
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