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首页> 外国专利> METHOD OF SURFACE STERILISATION OF EXPLANTS AND APICAL BUDS OF PINE STRAWBERRY, GRAPE, PERSIMMON OF VARIETY 'KOROLEK' IN VITRO

METHOD OF SURFACE STERILISATION OF EXPLANTS AND APICAL BUDS OF PINE STRAWBERRY, GRAPE, PERSIMMON OF VARIETY 'KOROLEK' IN VITRO

机译：松树莓，葡萄，柿子品种“ Korolek”的胚芽和大芽的表面灭菌方法

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FIELD: agriculture.;SUBSTANCE: invention relates to biotechnology and is a method of surface sterilisation of explants and apical buds of pine strawberry, grape, persimmon of variety "Korolek" in vitro. The method comprises sterilisation of explants and apical buds with 0.5% chlorhexidine alcoholic solution for five minutes and washing 5-6 times in sterile distilled water for 1 hour. Then from the explants the lamina are excised with the size larger than 4 mm and the notches are made to increase the area of contact of plant tissue with the nutrient medium. Then the apical buds are cleaned from the damaged tissues, and to determine purity of sterilisation they are placed on sterile agar nutrient medium. The medium contains 1/2 concentrations of macro- and micro-salts according to Murashige and Skoog (MS), the complete formulation of vitamins and organic additives of the medium MS, 1.0-2.0% sucrose, 0.50-0.70 mM 1-naphthyl acetic acid (NAA).;EFFECT: method enables to increase the reproduction factor of plants which are genetically stable and free from infections under in vitro conditions.;5 dwg, 1 tbl, 3 ex
机译：技术领域：本发明涉及生物技术，并且是对松树莓，葡萄，品种“ Korolek”的柿子的外植体和顶芽的表面进行体外灭菌的方法。该方法包括用0.5％氯己定醇溶液将外植体和顶芽灭菌5分钟，并在无菌蒸馏水中洗涤5-6次，持续1小时。然后从外植体上切下大于4 mm的叶片，并切出凹口以增加植物组织与营养培养基的接触面积。然后将根尖芽从受损的组织中清除，并确定灭菌的纯度，将其放在无菌琼脂营养培养基上。根据Murashige和Skoog（MS），该培养基包含1/2浓度的大盐和微盐，该培养基MS的维生素和有机添加剂的完整配方，1.0-2.0％蔗糖，0.50-0.70 mM 1-萘乙酸酸（NAA）。;效果：该方法能够提高在体外条件下遗传稳定且不受感染的植物的繁殖因子。5dwg，1 tbl，3 ex

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