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METHOD FOR PREPARING HIGHLY PURIFIED PREPARATION OF CERULOPLASMIN FERROXIDASE AND/OR BLOOD COAGULATION FACTOR PROTHROMBIN, AFFINE NEOMYCINE SORBENT FOR PREPARING THEM
METHOD FOR PREPARING HIGHLY PURIFIED PREPARATION OF CERULOPLASMIN FERROXIDASE AND/OR BLOOD COAGULATION FACTOR PROTHROMBIN, AFFINE NEOMYCINE SORBENT FOR PREPARING THEM
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机译：纯化血浆铜铁蛋白铁氧合酶和/或凝血因子凝血酶原的制备方法，仿射神经氨酸吸附剂的制备方法
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摘要
FIELD: medicine, pharmaceutics.;SUBSTANCE: substance of the invention is a method for recovering the preparations of ceruloplasmin (CP) and prothrombin (FIT) of human and mammalian blood plasma by two sequential chromatographies on anion sorbents, UNOQ-Sphere and neomycine agarose. UNOQ-Sphere contains -(+)N(CH3)3 - groups, and the affine sorbent contains neomycine agarose synthetised by the authors on the basis of the antibiotic neomycine a molecule of which contains 6NH2-groups. The prepared highly purified preparation of CP contains no FII and active thrombin (Flla), main proteinase causing proteoclastic degradation of CP. The preparation of CP contains up to 90% of non-degraded 132-kDa protein, characterised by a high criterion of purity A610/A280 - 0.052 and contains 5.9±0.1 mole of copper per a mole of CP. The above preparation of CP is stable for 2 months if stored aseptically even at 37°C. Preparing the pure end product CP of neomycine agarose is accompanied by recovering FII to be used as a raw material for preparing the haemostatic Flla.;EFFECT: advantage of the declared method represents no need for dissolving initial plasma at the first stage of the recovery, a short overall length of the recovery being 1-2 days, and a possibility to using the preparation in practice for scientific application and pharmaceutical industry.;2 cl, 4 dwg, 3 tbl, 6 ex
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