首页> 外国专利> METHOD FOR QUANTITATIVE ANALYSIS OF SPECIFIC IMMUNOGLOBULINS G TO CONJUGATE OF FORMALDEHYDE-SERUM HUMAN ALBUMIL IN BLOOD SERUM

METHOD FOR QUANTITATIVE ANALYSIS OF SPECIFIC IMMUNOGLOBULINS G TO CONJUGATE OF FORMALDEHYDE-SERUM HUMAN ALBUMIL IN BLOOD SERUM

机译:特定免疫球蛋白G与血液中甲醛和人血清白蛋白结合的定量分析方法

摘要

FIELD: medicine.;SUBSTANCE: when implementing the method for quantitative analysis of specific immunoglobulins G to a conjugate of formaldehyde - human serum albumin in blood serum, test and reference nitro-cellulose substrate are prepared by placing them in various microplate wells with said test substrate prepared by sequential sorption of human serum albumin and formaldehyde thereon at room temperature for 8 hours, while the reference substrate is prepared by sorption of human serum albumin thereon. It is followed by incubation of the test and reference substrates with the analysed serum sample for 30 minutes. After the substrates are washed in a phosphate buffer, the substrates are added with monoclonal antibodies with Fc-fragments of human immunoglobulin G conjugated with horseradish peroxidase; the substrates are washed in the phosphate buffer once more, and an immunochemical complex of specific immunoglobulin G antibodies are coloured by adding chromogene and developed by adding a stop reagent, The worked out substrates are removed from the microplate wells, and optical density is recorded in the test and reference samples; they are matched with optical density of the reference samples of the known concentration of immunoglobulin G, and a difference of the content of immunoglobulin G in the test and reference samples shows a number of patient's blood serum immunoglobulin G specific to the test substance provided optical density in the test sample exceeding that of the reference sample not less than 1.5 times.;EFFECT: method enables higher accuracy and reliability of determining specific immunoglobulins G in blood serum.;2 tbl, 1 ex
机译:领域:药物:物质:当实施定量分析特异性免疫球蛋白G与血清中的甲醛-人血清白蛋白结合物的方法时,将测试和参考硝化纤维素底物置于上述测试的各种微孔板中进行制备通过在室温下将人血清白蛋白和甲醛连续吸附8小时制得的底物,而通过将人血清白蛋白吸附在其上制备参考底物。随后将测试和参考底物与分析的血清样品一起孵育30分钟。在磷酸盐缓冲液中洗涤底物后,向底物中添加单克隆抗体,该单克隆抗体具有与辣根过氧化物酶偶联的人免疫球蛋白G的Fc片段。再次在磷酸盐缓冲液中洗涤底物,并通过添加显色剂对特定免疫球蛋白G抗体的免疫化学复合物进行着色,并通过添加终止试剂进行显影,将已处理的底物从微孔板孔中移出,并记录光密度。测试和参考样品;它们与已知浓度的免疫球蛋白G的参比样品的光密度相匹配,并且测试样品和参比样品中免疫球蛋白G含量的差异显示了特定于被测物质的患者的血清免疫球蛋白G所提供的光密度测试样品中的总免疫球蛋白含量超过参考样品的1.5倍;效果:该方法可以测定血清中特定免疫球蛋白G的准确性和可靠性更高; 2 tbl,1 ex

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