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Production of Seonghwan-commercial chicken No. 1 using Korean native chicken and exotic chicken and distinction method of the product

机译:使用韩国本地鸡和外来鸡生产成城商业鸡1号及其产品区分方法

摘要

PURPOSE: A method for producing Seonghwan-commercial chicken number one and a method for distinguishing the product are provided to improve the quality of the product and to obtain peculiar genetic properties. CONSTITUTION: A method for producing Seonghwan-commercial chicken number one is as follows. Genomic DNA is extracted from hen species to be analyzed. PCR(Polymerase Chain Reaction) is performed by using a primer pair of the sequence number 3 and 4. The insertion of the sequence number 1 and 2 is confirmed from the PCR amplification product. PCR is performed by using the primer pair of the sequence number 5 and 6. The restriction fragment length polymorphism analysis is performed by using Alu I restriction enzyme.
机译:目的:提供一种生产城焕商业鸡的方法和一种区分产品的方法,以提高产品的质量并获得独特的遗传特性。组成:成城商业鸡一号的生产方法如下。从母鸡物种中提取基因组DNA进行分析。通过使用序列号3和4的引物对进行PCR(聚合酶链反应)。从PCR扩增产物确认序列号1和2的插入。通过使用序列号5和6的引物对进行PCR。通过使用Alu I限制酶进行限制性片段长度多态性分析。

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