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METHOD FOR CORRECTING A CARBON DIOXIDE MEASURING MICROELECTRODE, AN OXYGEN MEASURING MICROELECTRODE, A PH MEASURING MICROELECTRODE PROPER TO A STUDY ON EFFECTS OF THE PRIMARY PRODUCTION OF BENTHIC MICROALGAE LIVE ON THE SEA BOTTOM
METHOD FOR CORRECTING A CARBON DIOXIDE MEASURING MICROELECTRODE, AN OXYGEN MEASURING MICROELECTRODE, A PH MEASURING MICROELECTRODE PROPER TO A STUDY ON EFFECTS OF THE PRIMARY PRODUCTION OF BENTHIC MICROALGAE LIVE ON THE SEA BOTTOM
PURPOSE: A method for correcting a carbon dioxide measuring microelectrode, an oxygen measuring microelectrode, a pH measuring microelectrode is proper to a study on effects of the primary production of benthic microalgae live on the sea bottom.;CONSTITUTION: A method for correcting a carbon dioxide measuring microelectrode, an oxygen measuring microelectrode, a pH measuring microelectrode includes a correction method using carbonate solution and a correction method using reference carbon dioxide gas. The correction method using carbonate solution is as follows. 200 mM NaHCO3 solution at predetermined low concentration is successively putted to the microelectrodes by using 50 mM phosphate buffer in which the fresh water of pH 7.2 and the sea water of pH 8.2 are mixed. The 50 mM phosphate buffer in which the fresh water of pH 7.2 and the sea water of pH 8.2 are mixed is heated and cooled under a vacuum condition after removing carbon dioxide and oxygen as much as possible within a predetermined range. Nitrogen gas is injected into the 50 mM phosphate buffer so that the carbon dioxide becomes zero. A blank is measured, and 10 ml of 200 mM NaHCO3 solution is added to the 50 mM phosphate buffer. When mV becomes a value within a predetermined range, the mV is recorded. 20 ml, 40ml, 80ml, 160ml, 320ml, 640ml, and 1280 ml of 200 mM NahCO3 solution are successively added to the 50 mM phosphate buffer, and the blanks are successively measured.;COPYRIGHT KIPO 2013;[Reference numerals] (AA) Start; (BB) End; (S110) 50 mM phosphate buffer is heated and cooled under a vacuum condition after removing carbon dioxide and oxygen as much as possible within a predetermined range; (S120) Nitrogen gas is injected into the 50 mM phosphate buffer so that the carbon dioxide becomes zero; (S130) Blank is measured, 10 ml of 200 mM NaHCO3 solution is added, and mV is recorded when the mV becomes a value within a predetermined range; (S140) 20 ml, 40ml, 80ml, 160ml, 320ml, 640ml, and 1280 ml of 200 mM NahCO3 solution are successively added, and the blanks are successively measured
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机译：目的：一种校正二氧化碳测量微电极，一种氧气测量微电极，一种pH测量微电极的方法，适合于研究海底生活的底层微藻的初级生产的影响。;构成：一种校正碳的方法二氧化碳测定用微电极，氧测定用微电极，pH测定用微电极包括使用碳酸盐溶液的校正方法和使用基准二氧化碳气体的校正方法。使用碳酸盐溶液的校正方法如下。通过使用50 mM磷酸盐缓冲液，将200 mM NaHCO3溶液以预定的低浓度依次放入微电极，其中将pH 7.2的淡水和pH 8.2的海水混合。在预定范围内尽可能多地除去二氧化碳和氧气之后，在真空条件下加热和冷却其中混合有pH 7.2的淡水和pH 8.2的海水的50 mM磷酸盐缓冲液。将氮气注入50 mM磷酸盐缓冲液中，以使二氧化碳变为零。测量空白，并将10 ml 200 mM NaHCO3溶液添加到50 mM磷酸盐缓冲液中。当mV变为预定范围内的值时，记录mV。向50 mM磷酸盐缓冲液中依次添加20 ml，40 ml，80 ml，160 ml，320 ml，640 ml和1280 ml 200 mM NahCO3溶液，并连续测量空白。; COPYRIGHT KIPO 2013; [参考数字]（AA）开始; （BB）结束; （S110）在预定范围内尽可能地除去二氧化碳和氧气后，在真空条件下加热和冷却50 mM磷酸盐缓冲液; （S120）将氮气注入50mM磷酸盐缓冲液中，以使二氧化碳变为零; （S130）测量空白，加入10ml 200mM NaHCO 3溶液，当mV变为预定范围内的值时记录mV。 （S140）依次添加20 ml，40 ml，80 ml，160 ml，320 ml，640 ml和1280 ml 200 mM NahCO3溶液，并连续测量空白
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