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METHOD FOR DETECTING AND IDENTIFYING QUINOLONE RESISTANT CAMPYLOBACTER FROM BIOLOGICAL SAMPLE USING POLYMERASE CHAIN REACTION AND KIT USED FOR THE SAME METHOD
METHOD FOR DETECTING AND IDENTIFYING QUINOLONE RESISTANT CAMPYLOBACTER FROM BIOLOGICAL SAMPLE USING POLYMERASE CHAIN REACTION AND KIT USED FOR THE SAME METHOD
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机译：聚合酶链反应从生物样品中检测抗喹诺酮类细菌的方法及用于同一方法的试剂盒
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摘要
PURPOSE: A method for detecting a quinolone resistant Campylobacter variant in a biological sample and a primer set and a kit for PCR for the same are provided to cheaply and simply detect and distinguish quinolone resistant variants of C. jejuni and C.coli. CONSTITUTION: A method for distinguishing and detecting a wild type and a quinolone resistant variant type of C. jejuni and C.coli in a biological sample comprises: a step of isolating DNA from the biological sample; a step of performing PCR using the DNA as a template and a primer set for PCR containing an oligonucleotide of sequence numbers 1, 2, 3, 4, 5, and 6; and a step of analyzing a PCR product and distinguishing wild type and variant type.
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