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Mutant Sensory Rhodopsin with Altered Proton Pumping activity
Mutant Sensory Rhodopsin with Altered Proton Pumping activity
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机译:质子泵浦活性改变的突变感官视紫红质
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摘要
the present invention is that the cells inside the proton pumping ability improved sensor rhodopsin mutations Ana Vena (Anabaena Sensor rhodopsin; ASR) relates to a protein , more specifically, SEQ ID NO: 1 amino acid mutation is the 217th Asp in wild-type rhodopsin Ana vena sensor comprising the amino acid sequence , characterized in that the substrate is replaced by Glu to know vena sensor rhodopsin and hydrogen ions using the same. relates to a pumping system . Because mutant rhodopsin Ana vena sensor protein of the present invention has the ability to pump the hydrogen ions into the cell by the light irradiation , the membrane potential of control with other pumping rhodopsin to pump protons into the cell outside can be used very usefully . ; 展开▼
机译:本发明是质子泵能力内的细胞改善了传感器视紫红质的突变Ana Vena(Anabaena Sensor rhodopsin; ASR)与一种蛋白质有关,更具体地讲,SEQ ID NO:1氨基酸突变是野生型视紫红质中的第217位包含氨基酸序列的模拟腔传感器,其特征在于,将底物替换为Glu以了解腔传感器视紫红质和氢离子。涉及抽水系统。由于本发明的突变视紫红质Ana vena传感器蛋白具有通过光照射将氢离子泵入细胞的能力,因此利用其他泵浦视紫红质将质子泵入细胞外的控制膜电位是非常有用的。 ; 展开▼