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METHOD FOR IDENTIFICATION OF ONCOGENE, METHOD FOR ESTABLISHMENT OF CELL CAPABLE OF EXPRESSING ONCOGENE, AND METHOD FOR SCREENING FOR ONCOGENE-TARGETING SUBSTANCE
METHOD FOR IDENTIFICATION OF ONCOGENE, METHOD FOR ESTABLISHMENT OF CELL CAPABLE OF EXPRESSING ONCOGENE, AND METHOD FOR SCREENING FOR ONCOGENE-TARGETING SUBSTANCE
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机译:致癌基因的鉴定方法,可表达致癌基因的细胞的建立方法以及致癌基因靶向物质的筛选方法
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摘要
The present invention provides a methodology that can develop a more excellent anti-cancer drug than conventional ones for various cancers. Specifically, the present invention provides a method for establishing an artificial cell, comprising treating a cancer cell with an expression vector for a foreign oncogene and then culturing the cancer cell treated with the expression vector under a condition which inhibits an expression or function of an oncogene that is inherent to the cancer cell, and an established artificial cell; a method of screening an anti-cancer drug, comprising evaluating whether or not a test substance inhibits a proliferation of the artificial cell; as well as a method for identifying an oncogene, comprising treating a cancer cell with an expression vector for a test gene and then culturing the cancer cell treated with the expression vector under a condition which inhibits an expression or function of an oncogene that is inherent to the cancer cell.
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