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Procedure for the undifferentiated or myeloid lineage biased expansion of haematopoietic stem cells from umbilical cord blood, mobilized peripheral blood or bone marrow
Procedure for the undifferentiated or myeloid lineage biased expansion of haematopoietic stem cells from umbilical cord blood, mobilized peripheral blood or bone marrow
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机译：从脐带血，动员的外周血或骨髓中造血干细胞的未分化或髓系谱系偏向扩增的程序
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摘要
Procedure for the undifferentiated or myeloid lineage biased expansion of haematopoietic stem cells coming from umbilical cord blood, mobilized peripheral blood or bone marrow.;Procedure for the undifferentiated or myeloid lineage biased expansion of haematopoietic stem cells (HSCs). More specifically, the present invention relates to a procedure of expansion of HSCs from umbilical cord blood, bone marrow or mobilized peripheral blood. Said procedure comprises the steps of expansion culturing of the purified CD34+ cells at constant volume, expansion culturing of said cells at variable volume and the conditioning of the CD34+ cells for transplantation. With this procedure the dose of undifferentiated or myeloid lineage biased HSCs which is necessary for their clinical use is produced reproducibly, robustly and safely.
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