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CONSTRUCTION METHOD OF ESCHERICHIA COLI GENETICALLY ENGINEERED BACTERIA PRODUCING SUCCINIC ACID BY XYLOSE METABOLISM

机译：木糖代谢制备大肠杆菌酸性大肠杆菌的基因工程菌的构建方法

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摘要

Provided is a construction method of an escherichia coli genetically engineered bacteria producing succinic acid by xylose metabolism and the method for producing succinic acid by fermentation using the bacteria. The ATP biosynthesis pathway of escherichia coli is modified by means of molecular biology and the enzyme activity related to the pathway is over-expressed; thus the total ATP amount within escherichia coli cells is effectively increased such that the recombinant escherichia coli is able to grow using xylose metabolism, and the succinic acid synthesis efficiency is thereby significantly improved.

机译：本发明提供了一种通过木糖代谢产生琥珀酸的大肠杆菌基因工程菌的构建方法以及通过使用该细菌的发酵产生琥珀酸的方法。大肠杆菌的ATP生物合成途径是通过分子生物学方法进行修饰的，与该途径有关的酶活性过表达。因此，有效增加了大肠杆菌细胞内的总ATP量，使得重组大肠杆菌能够利用木糖代谢生长，从而显着提高了琥珀酸的合成效率。

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公开/公告号WO2013010483A1

专利类型
公开/公告日2013-01-24

原文格式PDF
申请/专利权人 NANJING UNIVERSITY OF TECHNOLOGY;JIANG MIN;LIU RONGMING;LIANG LIYA;MA JIANGFENG;CHEN KEQUAN;WEI PING;
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申请/专利号WO2012CN78817
发明设计人 JIANG MIN;LIU RONGMING;LIANG LIYA;MA JIANGFENG;CHEN KEQUAN;WEI PING;
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申请日2012-07-18
分类号C12N15/70;C12P7/46;C12R1/19;
国家 WO
入库时间 2022-08-21 16:35:22

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