首页> 外国专利> COMPOSITION FOR SIMULTANEOUSLY DETECTING MYCOBACTERIUM TUBERCULOSIS AND NONTUBERCULOUS MYCOBACTERIA BY MEANS OF REAL-TIME MULTIPLEX POLYMERASE CHAIN REACTION COMPRISING NESTED HYBRIDIZATION PNA PROBE SYSTEM HAVING PARALLEL BINDING STRUCTURE, AND METHOD FOR DETECTION USING SAME

COMPOSITION FOR SIMULTANEOUSLY DETECTING MYCOBACTERIUM TUBERCULOSIS AND NONTUBERCULOUS MYCOBACTERIA BY MEANS OF REAL-TIME MULTIPLEX POLYMERASE CHAIN REACTION COMPRISING NESTED HYBRIDIZATION PNA PROBE SYSTEM HAVING PARALLEL BINDING STRUCTURE, AND METHOD FOR DETECTION USING SAME

机译:利用具有平行结合结构的嵌套多杂交PNA探针系统实时多重聚合酶链反应实时检测结核分枝杆菌和非结核分枝杆菌的方法及检测方法

摘要

The present invention relates to a composition for simultaneously detecting Mycobacterium tuberculosis and nontuberculous mycobacteria based on a nested hybridization peptide nucleic acid (PNA) probe system having a parallel binding structure, and to a method for simultaneously detecting Mycobacterium tuberculosis and nontuberculous mycobacteria using the composition and real-time polymerase chain reaction. Also, the present invention relates to a primer, a probe composition, and a method for analyzing to simultaneously detect Mycobacterium tuberculosis and nontuberculous mycobacteria based on the nested hybridization PNA probe system having the parallel binding structure. When carrying out the real-time multiplexed polymerase chain reaction by using the composition comprising the nested hybridized PNA probe system having the parallel binding structure, according to the present invention, Mycobacterium tuberculosis and nontuberculous mycobacteria can be simultaneously and accurately detected in a single tube with high sensitivity, thereby providing the advantage of making a more rapid and accurate clinical diagnosis.
机译:本发明涉及基于具有平行结合结构的嵌套杂交肽核酸(PNA)探针系统同时检测结核分枝杆菌和非结核分枝杆菌的组合物,并涉及使用该组合物同时检测结核分枝杆菌和非结核分枝杆菌的方法。实时聚合酶链反应。此外,本发明涉及基于具有平行结合结构的嵌套杂交PNA探针系统,同时检测结核分枝杆菌和非结核分枝杆菌的引物,探针组合物和分析方法。当通过使用包含具有平行结合结构的嵌套杂交PNA探针系统的组合物进行实时多重聚合酶链反应时,根据本发明,可以在单个管中同时准确地检测结核分枝杆菌和非结核分枝杆菌。高灵敏度,因此具有进行更快,更准确的临床诊断的优势。

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