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METHOD FOR EVALUATION OF ACUTE ORGAN TOXICITY OF COMPOUNDS OF HEAVY METALS IN VITRO IN THE MODEL OF HUMAN CELL CULTURE

机译:在人体细胞模型中评估重金属化合物的急性有机毒性的方法

摘要

Disclosed is a method for the determination of organ toxicity of compounds of heavy metals in vitro in model of cell culture for different concentrations of compositions of heavy metals. Cultures of cells of human organs are used as models for evaluation of acute organ toxicity of compounds of heavy metals in vitro, to nutrient medium of those compositions of heavy metals are added, by means of tests with methyl thiasolyl tetrazoium, neutral red and sulphorodamine B characteristics of cytotoxic effect and ECare determined, with comparison of those for different cultures, thus organospecific cell culture that is most sensitive to effect of the substance under investigation is found.
机译:公开了一种用于在细胞培养模型中针对不同浓度的重金属组成物体外测定重金属化合物的器官毒性的方法。人体器官细胞培养物用作评估重金属化合物体外急性器官毒性的模型,通过甲基噻唑基四唑鎓,中性红和磺酰罗丹明B的试验向重金属成分的营养培养基中添加通过与不同培养物进行比较,确定了细胞毒性作用和EC的特征,从而找到了对所研究物质的作用最敏感的有机特异性细胞培养物。

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