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METHOD FOR OBTAINING CALLUSE TISSUE IN FODDER BEET CULTURE IN VITRO
METHOD FOR OBTAINING CALLUSE TISSUE IN FODDER BEET CULTURE IN VITRO
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机译:体外获得甜菜文化中ALL愈组织的方法
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摘要
A method for obtaining callus tissue in fodder beet in culture in vitro comprises taking out sterile fodder beet explants, cultivating on Gamborg and Evelegs modified agarized nutrient medium, in the conditions of a thermostat in the course of 3 weeks. Floral shoots are sterilized, cultivation is carried out at a temperature of 24±, nonmature germs are used as an explant, Gamborg and Evelegs medium is used adding the following components: BAP 0.5 mg/l, gibberellic acid - 1.0 mg/l, NOK 1.0 mg/l, 2,4-D - 0.5-0.8 mg/l, glycine 2.0 mg/l, sucrose 30.0-50.0 mg/l.
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