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METHOD FOR OBTAINING CALLUSE TISSUE IN FODDER BEET CULTURE IN VITRO

机译:体外获得甜菜文化中ALL愈组织的方法

摘要

A method for obtaining callus tissue in fodder beet in culture in vitro comprises taking out sterile fodder beet explants, cultivating on Gamborg and Evelegs modified agarized nutrient medium, in the conditions of a thermostat in the course of 3 weeks. Floral shoots are sterilized, cultivation is carried out at a temperature of 24±, nonmature germs are used as an explant, Gamborg and Evelegs medium is used adding the following components: BAP 0.5 mg/l, gibberellic acid - 1.0 mg/l, NOK 1.0 mg/l, 2,4-D - 0.5-0.8 mg/l, glycine 2.0 mg/l, sucrose 30.0-50.0 mg/l.
机译:一种在体外培养的饲料甜菜中获得愈伤组织的方法,包括取出无菌的饲料甜菜外植体,在恒温器的条件下,在3周的时间内,在Gamborg和Evelegs改性的琼脂营养培养基上进行培养。将花芽灭菌,在24±20°C的温度下进行培养,使用未成熟的细菌作为外植体,使用Gamborg和Evelegs培养基添加以下成分:BAP 0.5 mg / l,赤霉素-1.0 mg / l,NOK 1.0 mg / l,2,4-D-0.5-0.8 mg / l,甘氨酸2.0 mg / l,蔗糖30.0-50.0 mg / l。

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