首页> 外国专利> PROCESS FOR ACTIVATING CELL PROLIFERATION WITH EFFECT ON REGENERATION OF BURNT AND WOUNDED TISSUE AND MATRIX METALLOPROTEINASE, BY STIMULATING CELL ADHESION AND DIFFERENTIATION UNDER THE ACTION OF LEVAN PRODUCED BY ZYMOMONAS MOBILI BACTERIA GROWN ON SWEET SORGHUM () EXTRACTS

PROCESS FOR ACTIVATING CELL PROLIFERATION WITH EFFECT ON REGENERATION OF BURNT AND WOUNDED TISSUE AND MATRIX METALLOPROTEINASE, BY STIMULATING CELL ADHESION AND DIFFERENTIATION UNDER THE ACTION OF LEVAN PRODUCED BY ZYMOMONAS MOBILI BACTERIA GROWN ON SWEET SORGHUM () EXTRACTS

机译：通过在甜食中生长的运动发酵单胞菌（细菌）产生的Levan刺激细胞粘附和分化，激活细胞增殖，从而影响创口和创面组织和基质金属蛋白酶的再生

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摘要

The present invention relates to a process for obtaining by fermentation a polysaccharide grown on extracts of sweet sorghum (Sorghum bicolor var. saccharum) standardized at 5% sucrose, without additions of mineral salts or yeast extract, with applications in the tissue and reparative engineering for regenerating burnt or wounded tissue and for reconstructing the adipose tissue. The process involves the treatment with a polyfructan (levan) solution produced by the Gram negative Zymomonas mobilis bacteria of the dermal cells and the adipogenic progenitors, respectively, the growth on solid and liquid medium, in the absence of some additions of mineral salts or growth factors, the treatment effect being controlled by the analysis of the tissue metal proteinase profile, adhesion and cell proliferation analyses as well as adipogenic differentiation which are performed by specific techniques, such as gelatin zymography, phase-contrast microscopy, fluorescence and spectrophotometry assays.

机译：本发明涉及通过发酵获得在不添加矿物盐或酵母提取物的情况下在不添加矿物质盐或酵母提取物的情况下在以5％蔗糖标准化的甜高粱（高粱）上提取的多糖的方法，其用于组织和修复工程中。再生烧伤或受伤的组织，并用于重建脂肪组织。该过程涉及用真皮细胞的运动革兰氏阴性运动发酵单胞菌细菌和成脂祖细胞分别产生的聚果聚糖溶液处理，在固体和液体培养基上的生长，而无需添加一些无机盐或生长。通过分析组织金属蛋白酶特征，粘附和细胞增殖分析以及成脂分化来控制治疗效果，这些是通过特定技术进行的，例如明胶酶谱，相差显微镜，荧光和分光光度法。

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公开/公告号RO128714A2

专利类型
公开/公告日2013-08-30

原文格式PDF
申请/专利权人 UNIVERSITATEA DIN BUCURESTI;
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申请/专利号RO20110001357
发明设计人 STURZOIU CRISTINA;STOIAN GHEORGHE;PETRESCU MARIA;GALATEANU BIANCA;DINISCHIOTU ANCA;COSTACHE MARIETA;
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申请日2011-12-08
分类号A61L27/20;A61L27/54;A61L27/60;
国家 RO
入库时间 2022-08-21 16:40:04

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