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SILVER NANO-PARTICLES SYNTHESIZED WITH THE HELP OF CELL-FREE EXTRACT OF HEMI-BIOTROPHIC FUNGUS MAGNAPORTHE ORYZAE(B. COUCH.) SP NOV., ITS USE AS ANTIBACTERIAL AND ANTIFUNGAL AGENT AND ALSO AS APOPTOGENIC AGENT.
SILVER NANO-PARTICLES SYNTHESIZED WITH THE HELP OF CELL-FREE EXTRACT OF HEMI-BIOTROPHIC FUNGUS MAGNAPORTHE ORYZAE(B. COUCH.) SP NOV., ITS USE AS ANTIBACTERIAL AND ANTIFUNGAL AGENT AND ALSO AS APOPTOGENIC AGENT.
• This invention involves the first reported procedure of synthesizing silver nanoparticles using hemibiotrophic fungus Magnaporthe oryzae (B. Couch sp. Nov) .The method for synthesizing silver nanoparticles involves the preparation of cell filtrate from mycelial mat from the above stated organism and further exposing it to 1 mM concentration of silver nitrate solution. The extracellular "green" synthesis of fungal enzymes and metabolites that are responsible for reduction of silver nitrate and produces silver nanoparticles with a capping agent. The range of silver nanoparticles thus produced is of diameter 2-26nm which are mainly spherical. • The invention also corroborates the characterization of silver nanoparticles in terms of dispersity, particle distribution, shapes and sizes. In this case the particle is monodispersive with little or no aggregation, generally spherical with average diameter of 8.6nm±2nm. • The silver nanoparticles thus produced exhibit its antioxidant property that is estimated according to the formation of phosphomolybdenum complex where M. oryzae derived silver nanoparticles (MONPs) showed higher total antioxidant capacity in comparison to cell filtrate and ascorbic acid. It has also been seen that MONPs have potent antimicrobial activity against multidrug resistant strains of bacteria of both plants and human. It also shows anti-fungal property against plant pathogenic fungi. Most importantly its role in terms of apoptogenic property is seen in case of eukaryotic cells by dosage dependent way, by comet assays. So, thereby it can be said that it is both economical and environmental friendly way to synthesize silver nanoparticles with application as antimicrobial agent, antitumor agent and also as means of plant disease control. • The reactions are conducted specifically at 28°C which is less than 90°C required in chemical synthesis methods. Therefore it can be used in both conductive coatings and adhesives. • These silver nanoparticles (MONPs) are naturally protein coated and thereby likely to improve eukaryotic cell uptake and retention for therapeutic purpose including but not restricted to drug delivery in tumor cells.
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